MTT assay of human anti-breast cancer cells (MCF-7) in vitro potentials and phytochemicals screening of the root bark extracts from Cassia sieberiana

IF 2 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Pure and Applied Chemistry Pub Date : 2024-02-14 DOI:10.1515/pac-2023-1108

Labaran Abdulkadir Magashi

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Abstract

Medicinal plant Cassia sieberiana root bark was investigated for phytochemicals and Anti-Breast Cancer cells (MCF-7) properties, using Chemical Separations and MTT assay in vitro. The C. sieberiana root bark was extracted with soxhlet extractor using different solvents based on polarity guided method and the respective extracts were concentrated under reduced pressure. These extracts were screened for their phytochemicals qualitatively using standard methods. Percentage Yields and Physico-Chemical Evaluation of the Extracts from the various fractions were recorded. The results of phytochemicals screening revealed the presence of some secondary metabolites of pharmacological significance in the Methyl acetate, MeOH and 70 % MeOH root bark extracts including Saponins, Quinones, Phenolic, Steroids, Tannins, Flavounoids, Terpenoids, Anthraquinones, Cardiac-glycosides, Alkaloids, Carbohydrates, Glycosides and Coumarins. Results obtained from MTT assay revealed that Extracts of MeOH and 70 % MeOH samples shown a certain degree of inhibition towards MCF-7 cell line. IC50 were calculated using Graph Pad Prism 6 and results shown that IC50 of 245.3 μg/ml and 239.6 μg/ml dose dependent inhibitions in MCF-7 cells respectively, compared to Oxaliplatin IC50 of 38.04 μg/ml. Therefore, 70 % MeOH sample with IC50 of 239.6 μg/ml have shown more potential of inhibiting breast cancer cell, MCF-7 cells line. The inhibition of the root bark is attributed to some of the phytochemicals present in the plant.

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MTT 人抗乳腺癌细胞（MCF-7）的体外潜力和决明根皮提取物的植物化学成分筛选

利用化学分离法和 MTT 体外检测法研究了药用植物 Cassia sieberiana 根皮的植物化学成分和抗乳腺癌细胞（MCF-7）的特性。根据极性引导法，使用不同的溶剂在索氏提取器中提取 C. sieberiana 根皮，并在减压下浓缩各自的提取物。采用标准方法对这些提取物进行植物化学成分的定性筛选。记录了各种馏分提取物的产量百分比和理化评价。植物化学成分筛选结果表明，乙酸甲酯、MeOH 和 70 % MeOH 根皮提取物中存在一些具有药理意义的次生代谢物，包括皂苷、醌类、酚类、类固醇、单宁、黄酮类、萜类、蒽醌类、强心甙类、生物碱类、碳水化合物类、苷类和香豆素类。MTT 试验结果表明，MeOH 和 70 % MeOH 样品的提取物对 MCF-7 细胞株有一定程度的抑制作用。使用 Graph Pad Prism 6 计算了 IC50，结果显示，与奥沙利铂的 IC50（38.04 μg/ml）相比，MCF-7 细胞的 IC50 分别为 245.3 μg/ml 和 239.6 μg/ml。因此，IC50 为 239.6 μg/ml 的 70 % MeOH 样品对乳腺癌细胞 MCF-7 细胞株的抑制潜力更大。根皮的抑制作用归因于植物中的一些植物化学物质。

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来源期刊

Pure and Applied Chemistry 化学-化学综合

CiteScore

4.00

自引率

0.00%

发文量

审稿时长

3-8 weeks

期刊介绍： Pure and Applied Chemistry is the official monthly Journal of IUPAC, with responsibility for publishing works arising from those international scientific events and projects that are sponsored and undertaken by the Union. The policy is to publish highly topical and credible works at the forefront of all aspects of pure and applied chemistry, and the attendant goal is to promote widespread acceptance of the Journal as an authoritative and indispensable holding in academic and institutional libraries.