{"title":"On-site rapid detection of Listeria monocytogenes in dairy products using smartphone-integrated device-assisted ratiometric fluorescent sensors","authors":"Hui Li, Yuwei Ren, Rui Jiao, Xiyan Zhang, Wenhua Zhao, Hanfang Chen, Danfeng Zhang, Yizhong Shen, Changqing Zhu, Yingwang Ye","doi":"10.1111/1471-0307.13045","DOIUrl":null,"url":null,"abstract":"<p>The contamination of <i>Listeria monocytogenes</i> (<i>L. monocytogenes</i>) in dairy products poses a significant risk to human health. It is urgent to develop on-site rapid strategy to reduce associated threats. We employed carbon dots (CDs) and silicon nanoparticles (SiNPs) as energy donors and acceptors respectively. The CDs and SiNPs were functionalised with vancomycin and aptamers correspondingly, leading to the establishment of ratiometric fluorescent sensor. With a portable smartphone-integrated device, the ratios of blue/green values from the colour images depended on <i>L. monocytogenes</i> concentrations for quantification. The sensors were demonstrated for monitoring <i>L. monocytogenes</i> in dairy products within 30 min.</p>","PeriodicalId":13822,"journal":{"name":"International Journal of Dairy Technology","volume":"77 2","pages":"403-414"},"PeriodicalIF":2.5000,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Dairy Technology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1471-0307.13045","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The contamination of Listeria monocytogenes (L. monocytogenes) in dairy products poses a significant risk to human health. It is urgent to develop on-site rapid strategy to reduce associated threats. We employed carbon dots (CDs) and silicon nanoparticles (SiNPs) as energy donors and acceptors respectively. The CDs and SiNPs were functionalised with vancomycin and aptamers correspondingly, leading to the establishment of ratiometric fluorescent sensor. With a portable smartphone-integrated device, the ratios of blue/green values from the colour images depended on L. monocytogenes concentrations for quantification. The sensors were demonstrated for monitoring L. monocytogenes in dairy products within 30 min.
期刊介绍：
The International Journal of Dairy Technology ranks highly among the leading dairy journals published worldwide, and is the flagship of the Society. As indicated in its title, the journal is international in scope.
Published quarterly, International Journal of Dairy Technology contains original papers and review articles covering topics that are at the interface between fundamental dairy research and the practical technological challenges facing the modern dairy industry worldwide. Topics addressed span the full range of dairy technologies, the production of diverse dairy products across the world and the development of dairy ingredients for food applications.
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