E-CADERIN, N-CADERIN, SLUG, SNAIL, and TWIST contribute to epithelial-mesenchymal transition in salivary gland tumors

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-02-13 DOI:10.1111/jop.13516

Dandara Andrade de Santana, Poliana Ramos Braga, Cláudia Malheiros Camillo-Coutinho, Valéria Souza Freitas, Patrícia Ramos Cury, Daniel Araki Ribeiro, Iguaracyra Barreto de Araújo, Flávia Caló de Aquino Xavier, Jean Nunes dos Santos

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引用次数: 0

Abstract

Background

Transcription factors are important in the epithelial-mesenchymal transition process and are possibly related to the development of a more invasive tumor phenotype. Thus, the objective of this study was to analyze the expression and identify the localization of cellular markers related to the epithelial-mesenchymal transition process in salivary gland tumors.

Study Design

The expression and localization of E-CADERIN, N-CADERIN, SLUG, SNAIL, and TWIST were evaluated, using immunohistochemistry, in 48 salivary gland tumors, being 17 pleomorphic adenomas (PA), 14 adenoid cystic carcinomas (ACC), and 17 mucoepidermoid carcinomas (MEC). these proteins were compared to clinical and histopathologic parameters. normal gland tissues were included for immunohistochemical comparisons.

Results

ACC and MEC cases showed higher expression of SNAIL compared to PA. MEC showed high expression of SLUG and TWIST. Low expression of N-CADHERIN, SNAIL, and TWIST in ACC was frequent in T3 and T4. High expression of TWIST in MEC was more frequent at age ≥ 40 years A positive correlation was only observed between N-cadherin/SNAIL in ACC, between SNAIL/TWIST in MEC, and between SLUG/TWIST in PA.

Conclusion

This study provided insight into EMT-related proteins (E-cadherin, N-cadherin, SNAIL, SLUG, and TWIST) and their contribution to the maintenance of morphogenesis and the development of the salivary gland tumors and showed a positive correlation among N-CADHERIN/SNAIL in ACC and SNAIL/TWIST in MEC.

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E-CADERIN、N-CADERIN、SLUG、SNAIL 和 TWIST 有助于唾液腺肿瘤的上皮-间质转化。

背景：转录因子在上皮-间质转化过程中非常重要，可能与更具侵袭性的肿瘤表型的形成有关。因此，本研究旨在分析唾液腺肿瘤中与上皮-间质转化过程相关的细胞标记物的表达并确定其定位：研究设计：采用免疫组化方法评估了48种唾液腺肿瘤中E-CADERIN、N-CADERIN、SLUG、SNAIL和TWIST的表达和定位情况，其中包括17种多形性腺瘤（PA）、14种腺样囊性癌（ACC）和17种粘液表皮样癌（MEC）：结果：ACC和MEC病例的SNAIL表达量高于PA。MEC显示SLUG和TWIST的高表达。ACC 中 N-CADHERIN、SNAIL 和 TWIST 的低表达常见于 T3 和 T4。仅在 ACC 中 N-cadherin/SNAIL、MEC 中 SNAIL/TWIST 和 PA 中 SLUG/TWIST 之间观察到正相关：本研究有助于深入了解 EMT 相关蛋白（E-cadherin、N-cadherin、SNAIL、SLUG 和 TWIST）及其对形态发生的维持和唾液腺肿瘤发生的贡献，并显示 ACC 中的 N-CADHERIN/SNAIL 与 MEC 中的 SNAIL/TWIST 之间存在正相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464