Co-colonization of methicillin-resistant Staphylococcus aureus and Candida spp. in children with malignancies.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

AMB Express Pub Date : 2024-02-13 DOI:10.1186/s13568-024-01667-7

Raziyeh Baharvand, Fatemeh Fallah, Parvaneh Jafari, Leila Azimi

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Abstract

This study aimed to evaluate the interaction between methicillin-resistant Staphylococcus aureus(MRSA) and Candida spp. in the oral cavity of children with malignancies under chemotherapy. We evaluated the expression level of Als3p and mecA in Candida spp. and MRSA strains in both single colonization and co-colonization condition. Oral and nasal samples were collected by dry sponge swabs in 10 ml of sterile phosphate-buffered saline. The MRSA and Candida spp. was confirmed using the PCR method and mecA and Als3p genes, respectively. The SYBR Green-based quantitative real-time PCR was used to evaluate the relative expression levels of mecA and Als3p genes in MRSA and Candida spp., respectively. The frequency of S. aureus in oral-only and nasal-only swab samples were 14.1% (n = 24/170). 58.3% (n = 14/24) and 29.2% (n = 7/24) of S. aureus isolated from oral and nasal samples were MRSA, respectively. Among Candida species, C. albicans (n = 28/170; 16.5%) had the highest frequency. The oral co-colonization of MRSA and Candida spp. was detected in 4.7% (n = 8/170) patients. The overall average of gene expression levels among all Candida spp. and MRSA isolates indicated that the mecA and Als3p genes expression increased six and two times in co-colonization conditions compared to single colonization conditions, respectively. Our findings revealed the importance of polymicrobial infection in clinical settings and stated that it is possible that Candida spp. facilitates the infection of S. aureus and can lead to systemic infection in co-colonized patients.

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恶性肿瘤患儿耐甲氧西林金黄色葡萄球菌和白色念珠菌的共定植。

本研究旨在评估正在接受化疗的恶性肿瘤患儿口腔中耐甲氧西林金黄色葡萄球菌（MRSA）与念珠菌属之间的相互作用。我们评估了单一定植和共定植条件下念珠菌属和 MRSA 菌株中 Als3p 和 mecA 的表达水平。在 10 毫升无菌磷酸盐缓冲盐水中用干海绵拭子采集口腔和鼻腔样本。分别使用 PCR 方法和 mecA 和 Als3p 基因确认 MRSA 和念珠菌属。采用基于 SYBR Green 的定量实时 PCR 技术分别评估了 MRSA 和念珠菌属中 mecA 和 Als3p 基因的相对表达水平。在纯口腔和纯鼻腔拭子样本中，金黄色葡萄球菌的检出率分别为 14.1%（n = 24/170）、58.3%（n = 14/170）和 58.3%（n = 14/170）。从口腔和鼻腔样本中分离出的金黄色葡萄球菌中，分别有 58.3%（n = 14/24）和 29.2%（n = 7/24）为 MRSA。在念珠菌中，白念珠菌（n = 28/170；16.5%）的出现频率最高。4.7%（n = 8/170）的患者口腔中检测到 MRSA 和念珠菌共定植。所有念珠菌属和 MRSA 分离物基因表达水平的总体平均值表明，与单一定植条件相比，共同定植条件下 mecA 和 Als3p 基因的表达量分别增加了 6 倍和 2 倍。我们的研究结果揭示了多微生物感染在临床环境中的重要性，并指出念珠菌属可能会促进金黄色葡萄球菌的感染，并导致共定植患者的全身感染。

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来源期刊

AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

7.20

自引率

2.70%

发文量

141

审稿时长

13 weeks

期刊介绍： AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.