Western blotting: evolution of an old analytical method to a new quantitative tool for biomarker measurements.

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2024-03-01 Epub Date: 2024-02-13 DOI:10.4155/bio-2023-0212

Carolina Owen, Kelly A Fader, Mohamed Hassanein

引用次数: 0

Abstract

Western blotting (WB) is a widely used laboratory technique for detecting specific proteins in biological matrices. Recent advances in antibody production, automation, gel and membrane manufacturing and highly sensitive detection platforms have transformed WB from a labor-intensive and qualitative method into a highly reproducible and quantitative assay suitable for biomarker detection. Despite these significant improvements in the capabilities and efficiency of WB, there remain challenges that hinder its widespread application as a research, diagnostic (in two-tiered assays like Lyme disease testing) and drug development tool. This article describes recent innovations introduced to WB methodology and the remaining challenges that prevent its wider adoption for biomarker measurements throughout the drug development process.

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Western 印迹法：从一种古老的分析方法演变为一种新的生物标志物定量测量工具。

Western blotting（WB）是一种广泛应用的实验室技术，用于检测生物基质中的特定蛋白质。近年来，随着抗体生产、自动化、凝胶和膜制造以及高灵敏度检测平台的不断进步，WB 已从一种劳动密集型的定性方法转变为一种适合生物标记物检测的可重复性高的定量检测方法。尽管 WB 的功能和效率有了这些重大改进，但仍存在一些挑战，阻碍了它作为研究、诊断（在莱姆病检测等双层检测中）和药物开发工具的广泛应用。本文介绍了 WB 方法学的最新创新，以及阻碍其在整个药物开发过程中广泛应用于生物标记物测量的其余挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.