Western blotting: evolution of an old analytical method to a new quantitative tool for biomarker measurements.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-01 Epub Date: 2024-02-13 DOI:10.4155/bio-2023-0212

Carolina Owen, Kelly A Fader, Mohamed Hassanein

引用次数: 0

Abstract

Western blotting (WB) is a widely used laboratory technique for detecting specific proteins in biological matrices. Recent advances in antibody production, automation, gel and membrane manufacturing and highly sensitive detection platforms have transformed WB from a labor-intensive and qualitative method into a highly reproducible and quantitative assay suitable for biomarker detection. Despite these significant improvements in the capabilities and efficiency of WB, there remain challenges that hinder its widespread application as a research, diagnostic (in two-tiered assays like Lyme disease testing) and drug development tool. This article describes recent innovations introduced to WB methodology and the remaining challenges that prevent its wider adoption for biomarker measurements throughout the drug development process.

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Western 印迹法：从一种古老的分析方法演变为一种新的生物标志物定量测量工具。

Western blotting（WB）是一种广泛应用的实验室技术，用于检测生物基质中的特定蛋白质。近年来，随着抗体生产、自动化、凝胶和膜制造以及高灵敏度检测平台的不断进步，WB 已从一种劳动密集型的定性方法转变为一种适合生物标记物检测的可重复性高的定量检测方法。尽管 WB 的功能和效率有了这些重大改进，但仍存在一些挑战，阻碍了它作为研究、诊断（在莱姆病检测等双层检测中）和药物开发工具的广泛应用。本文介绍了 WB 方法学的最新创新，以及阻碍其在整个药物开发过程中广泛应用于生物标记物测量的其余挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.