Preparation and characterization of protein-antioxidant complex pickering particles for high internal phase emulsions

IF 11 1区 农林科学 Q1 CHEMISTRY, APPLIED
Jingjing Ding , Xiaoyuan Qiu , Yunxing Li, Ying Wang, Yi Mao, Cheng Yang, Yajuan Sun
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Abstract

Herein, novel Pickering particles were fabricated by integrating tannic acid (TA) into Spirulina protein isolates (SPI) to enhance the oxidative stability of Pickering high internal phase emulsions (Pickering-HIPEs). The interactions between SPI and TA were examined using molecular docking simulation, attenuated total reflection Fourier-transform infrared (ATR-FTIR), and isothermal titration calorimetry (ITC). The interfacial properties of SPI-TA (ST) complex particles with different ratios of SPI to TA were characterized, and their emulsifying performances were also investigated with different oil volume fractions. The results revealed that hydrogen bonds may play a key role in the formation of ST complex particles. The presence of TA improved the wettability of SPI, and the resultant ST complex particles displayed a better emulsifying capacity, with the ability to emulsify up to 85 vol% of oil. Especially, ST complex particles stabilized Pickering-HIPEs exhibited a narrower size distribution, a stronger gel network, better stability upon long-term storage and UV exposure. Additionally, the oxidative stability of embedded oil and lipophilic bioactive compounds in Pickering-HIPEs was significantly improved regardless of exposure to room temperature or UV irradiation. Our research findings are expected to provide novel antioxidant Pickering particles for food-grade Pickering-HIPEs.

Abstract Image

用于高内相乳液的蛋白质-抗氧化剂复合物剔除颗粒的制备与表征
本文通过将单宁酸(TA)融入螺旋藻蛋白质分离物(SPI)来制造新型皮克林颗粒,以增强皮克林高内相乳液(Pickering-HIPEs)的氧化稳定性。采用分子对接模拟、衰减全反射傅立叶变换红外光谱(ATR-FTIR)和等温滴定量热法(ITC)对 SPI 和 TA 之间的相互作用进行了研究。对不同 SPI 与 TA 比例的 SPI-TA(ST)复合物颗粒的界面特性进行了表征,并研究了它们在不同油体积分数下的乳化性能。结果表明,氢键可能在 ST 复合粒子的形成过程中起到了关键作用。TA 的存在改善了 SPI 的润湿性,由此产生的 ST 复合物粒子具有更好的乳化能力,可乳化高达 85% 的油。特别是稳定了 Pickering-HIPEs 的 ST 复合物颗粒,其粒度分布更窄,凝胶网络更强,在长期储存和紫外线照射下具有更好的稳定性。此外,无论暴露在室温还是紫外线照射下,Pickering-HIPEs 中嵌入的油和亲油生物活性化合物的氧化稳定性都得到了显著提高。我们的研究成果有望为食品级 Pickering-HIPE 提供新型抗氧化 Pickering 颗粒。
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来源期刊
Food Hydrocolloids
Food Hydrocolloids 工程技术-食品科技
CiteScore
19.90
自引率
14.00%
发文量
871
审稿时长
37 days
期刊介绍: Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication. The main areas of interest are: -Chemical and physicochemical characterisation Thermal properties including glass transitions and conformational changes- Rheological properties including viscosity, viscoelastic properties and gelation behaviour- The influence on organoleptic properties- Interfacial properties including stabilisation of dispersions, emulsions and foams- Film forming properties with application to edible films and active packaging- Encapsulation and controlled release of active compounds- The influence on health including their role as dietary fibre- Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes- New hydrocolloids and hydrocolloid sources of commercial potential. The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.
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