Progress on the quality control technology of next generation sequencing library.

Q3 Medicine

遗传 Pub Date : 2024-02-20 DOI:10.16288/j.yczz.23-262

Meng-Nan Cui, Yan Guo, Ya-Rong Wu, Guang-Qian Pei, Yu-Jun Cui

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引用次数: 0

Abstract

As a key supporting technology in the fields of life sciences and medicine, high-throughput sequencing has developed rapidly and become increasingly mature. The workflow of this technology can be divided into nucleic acid extraction, library construction, sequencing, and data analysis. Among these, library construction is a pivotal step that bridges the previous and subsequent stages. The effectiveness of library construction is contingent on the quality of upstream samples and also impacts the data analysis following sequence data output. The selection and implementation of library construction quality control techniques are crucial for enhancing the reliability of results and reducing errors in sequencing data. This review provides an in-depth discussion of library construction quality control techniques, summarizing and evaluating their principles, advantages and disadvantages, and applicability. It also discusses the selection of relevant technologies in practical application scenarios. The aim is to offer theoretical foundations and references for researchers, disease prevention and control personnel, and others when choosing library quality control techniques, thereby promoting the quality and efficiency of high-throughput sequencing work.

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新一代测序文库质量控制技术的进展。

作为生命科学和医学领域的关键支撑技术，高通量测序技术发展迅速，日趋成熟。该技术的工作流程可分为核酸提取、文库构建、测序和数据分析。其中，文库构建是衔接前后阶段的关键步骤。文库构建的效果取决于上游样本的质量，同时也影响到序列数据输出后的数据分析。选择和实施文库构建质量控制技术对提高结果可靠性和减少测序数据误差至关重要。本综述深入探讨了文库构建质量控制技术，总结并评估了其原理、优缺点和适用性。它还讨论了在实际应用场景中如何选择相关技术。旨在为科研人员、疾病防控人员等在选择文库质量控制技术时提供理论依据和参考，从而提高高通量测序工作的质量和效率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

遗传 Medicine-Medicine (all)

CiteScore

2.50

自引率

0.00%

发文量

6699

期刊介绍： Hereditas is a national academic journal sponsored by the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences and the Chinese Society of Genetics and published by Science Press. It is a Chinese core journal and a Chinese high-quality scientific journal. The journal mainly publishes innovative research papers in the fields of genetics, genomics, cell biology, developmental biology, biological evolution, genetic engineering and biotechnology; new technologies and new methods; monographs and reviews on hot issues in the discipline; academic debates and discussions; experience in genetics teaching; introductions to famous geneticists at home and abroad; genetic counseling; information on academic conferences at home and abroad, etc. Main columns: review, frontier focus, research report, technology and method, resources and platform, experimental operation guide, genetic resources, genetics teaching, scientific news, etc.