Comprehensive analysis of diel rhythmic expression of the medaka toll-like receptor gene family

IF 2.7 3区农林科学 Q1 FISHERIES

Developmental and comparative immunology Pub Date : 2024-02-09 DOI:10.1016/j.dci.2024.105143

Takahiko Hata , Hidetoshi Shimawaki , Suzuka Setoguchi , Natsuki Morimoto , Jun-ichi Hikima , Masahiro Sakai , Tomoya Kono

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引用次数: 0

Abstract

Several immune-related genes, including Toll-like receptors (TLR), are associated with circadian rhythms in mammals. However, information on the circadian rhythmic expression of TLRs in fish is limited. In this study, we aimed to analyze the regulation of diel oscillations in the expression of TLR genes in Japanese medaka (Oryzias latipes). The expression analysis revealed diel expression patterns of tlr1, tlr5m, tlr21, and clock genes (bmal1 and clock1) under a 12 h light:12 h dark cycle. The clock gene response element (E-box) was identified in the transcriptional regulatory regions of tlr1, tlr5m, and tlr21. Moreover, overexpressed bmal1 and clock1 enhanced expression levels of tlr1, tlr5m, and tlr21 in medaka embryo (OLHdrR-e3) cells. The expression of tlr1, tlr5m, and tlr21 was significantly decreased in OLHdrR-e3 after generating a bmal1 knockdown using a morpholino oligo. These results indicate the regulation of the diel rhythmic expression of several fish TLRs by clock genes.

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青鳉收费样受体基因家族昼夜节律表达的综合分析

包括 Toll 样受体（TLR）在内的一些免疫相关基因与哺乳动物的昼夜节律有关。然而，有关鱼类中 TLRs 的昼夜节律表达的信息却很有限。本研究旨在分析日本青鳉（Oryzias latipes）中 TLR 基因表达的昼夜振荡调控。表达分析揭示了在 12 小时光照：12 小时黑暗循环下 tlr1、tlr5m、tlr21 和时钟基因（bmal1 和 clock1）的昼夜表达模式。在 tlr1、tlr5m 和 tlr21 的转录调控区发现了时钟基因的反应元件（E-box）。此外，在青鳉胚胎（OLHdrR-e3）细胞中，过表达bmal1和clock1可提高tlr1、tlr5m和tlr21的表达水平。使用吗啉寡聚物敲除 bmal1 后，tlr1、tlr5m 和 tlr21 在 OLHdrR-e3 细胞中的表达量显著下降。这些结果表明，多种鱼类 TLRs 的昼夜节律表达受时钟基因的调控。

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来源期刊

Developmental and comparative immunology 医学-动物学

CiteScore

6.20

自引率

6.90%

发文量

206

审稿时长

49 days

期刊介绍： Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.