A Postbiotic Formulation of Lactiplantibacillus plantarum CRL 759 Attenuates Endotoxin Induced Uveitis.

IF 2.6 4区医学 Q2 OPHTHALMOLOGY

Ocular Immunology and Inflammation Pub Date : 2024-11-01 Epub Date: 2024-02-09 DOI:10.1080/09273948.2024.2310173

Bárbara Ivana Layús, María Alejandra Gómez, Silvia Inés Cazorla, Ana Virginia Rodriguez

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引用次数: 0

Abstract

Purpose: To evaluate the anti-inflammatory activity of a cell-free supernatant from Lactiplantibacillus plantarum CRL 759, in phosphate buffer modified according to Sorensen called POF-759.

Methods: The activity of POF-759 administered by means of eye drops was evaluated on animals subcutaneously injected with the lipopolysaccharide animals in which uveitis was induced by a subcutaneous injection of lipopolysaccharide (EIU). Clinical signs of ocular inflammation, cytokines and proteins were examined in the aqueous humor. Additionally, cellular infiltration was evaluated by histopathological analysis.

Results: The new postbiotic administered locally decreases signs of ocular damage, the number of infiltrating cells in the anterior and posterior chambers, the proinflammatory mediators and the proteins in the aqueous humor on mice with EIU.

Conclusions: Our results provide an impetus to relieve ocular inflammation and to identify and develop preventive and therapeutic approaches, to avoid deterioration and to maintain healthy eyes on inflammatory processes.

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植物乳杆菌 CRL 759 后益生菌制剂可减轻内毒素诱发的葡萄膜炎。

目的：评估植物乳杆菌（Lactiplantibacillus plantarum CRL 759）在根据索伦森（Sorensen）改良的磷酸盐缓冲液中的无细胞上清液（称为 POF-759）的抗炎活性：在皮下注射脂多糖（EIU）诱发葡萄膜炎的动物身上评估了通过滴眼液给药的 POF-759 的活性。对眼部炎症的临床症状、水样物质中的细胞因子和蛋白质进行了检测。此外，还通过组织病理学分析评估了细胞浸润情况：结果：局部给药的新型后生物制剂可减少眼损伤的迹象、前后房浸润细胞的数量、EIU 小鼠眼房水中的促炎介质和蛋白质：我们的研究结果为缓解眼部炎症、确定和开发预防和治疗方法、避免炎症恶化和维持眼睛健康提供了动力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ocular Immunology and Inflammation 医学-眼科学

CiteScore

6.20

自引率

15.20%

发文量

285

审稿时长

6-12 weeks

期刊介绍： Ocular Immunology & Inflammation ranks 18 out of 59 in the Ophthalmology Category.Ocular Immunology and Inflammation is a peer-reviewed, scientific publication that welcomes the submission of original, previously unpublished manuscripts directed to ophthalmologists and vision scientists. Published bimonthly, the journal provides an international medium for basic and clinical research reports on the ocular inflammatory response and its control by the immune system. The journal publishes original research papers, case reports, reviews, letters to the editor, meeting abstracts, and invited editorials.