ETV5 Silencing Produces Mesenchymal to Epithelial Transition in INS-1 (832/13) Cell Line.

IF 2 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Hormone and Metabolic Research Pub Date : 2024-03-01 Epub Date: 2024-02-09 DOI:10.1055/a-2246-4778

Yael Efrén Díaz-López, Vicenta Cázares-Domínguez, Francisco Arenas-Huertero, Ruth Gutierrez-Aguilar

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引用次数: 0

Abstract

ETV5 has been described to be involved in the epithelial to mesenchymal transition (EMT) mainly in cancer. It is known that EMT provokes cytoskeleton remodeling, improving cellular migratory, and invasive capabilities. Moreover, overexpression of ETV5 has been correlated to cancer development and this gene has been implicated in cell proliferation. However, little is known about the downregulation of ETV5 expression in a pancreatic cell line and the inverse mesenchymal to epithelial transition (MET). Therefore, we studied the implications of ETV5 silencing over the phenotype of the insulinoma INS-1 (832/13) cell line and described the MET by partial ETV5 silencing in the INS-1 (832/13) cell line. The downregulation of ETV5 expression was obtained by using ETV5 siRNA in the insulinoma rat cell line, INS-1 (832/13). Then, ETV5 knockdown provoked a MET phenotype observed by crystal violet staining and verified by immunohistochemistry against E-cadherin. Wound healing assay showed no migration, and F-actin stain revealed rearrangement of actin microfilaments. In addition, TGFβ1 and TGFβ3 were downregulated in the absence of ETV5. ETV5 silencing induces epithelial phenotype by downregulating TGFβ1 and TGFβ3 in INS-1 (832/13) cell line.

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沉默 ETV5 可使 INS-1 (832/13) 细胞系发生间充质向上皮的转化。

据描述，ETV5 参与了上皮向间充质转化（EMT），主要是在癌症中。众所周知，EMT 会引起细胞骨架重塑，改善细胞迁移和侵袭能力。此外，ETV5 的过表达与癌症的发展有关，而且该基因与细胞增殖有牵连。然而，人们对 ETV5 在胰腺细胞系中的表达下调以及间质向上皮的逆转变（MET）知之甚少。因此，我们研究了 ETV5 沉默对胰岛素瘤 INS-1 (832/13) 细胞系表型的影响，并描述了 INS-1 (832/13) 细胞系中部分 ETV5 沉默导致的 MET。在胰岛素瘤大鼠细胞系INS-1（832/13）中使用ETV5 siRNA可下调ETV5的表达。然后，通过水晶紫染色观察到 ETV5 基因敲除导致的 MET 表型，并通过针对 E-cadherin 的免疫组化进行验证。伤口愈合试验显示没有迁移，F-肌动蛋白染色显示肌动蛋白微丝重新排列。此外，在 ETV5 缺失的情况下，TGFβ1 和 TGFβ3 下调。沉默ETV5可通过下调TGFβ1和TGFβ3诱导INS-1（832/13）细胞系的上皮表型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hormone and Metabolic Research 医学-内分泌学与代谢

CiteScore

3.80

自引率

0.00%

发文量

125

审稿时长

3-8 weeks

期刊介绍： Covering the fields of endocrinology and metabolism from both, a clinical and basic science perspective, this well regarded journal publishes original articles, and short communications on cutting edge topics. Speedy publication time is given high priority, ensuring that endocrinologists worldwide get timely, fast-breaking information as it happens. Hormone and Metabolic Research presents reviews, original papers, and short communications, and includes a section on Innovative Methods. With a preference for experimental over observational studies, this journal disseminates new and reliable experimental data from across the field of endocrinology and metabolism to researchers, scientists and doctors world-wide.