Jamal James D. Manlapig, Suzuna Kawakami, Masaya Matamura, Makoto Kondo, Tomomi Ban-Tokuda, Hiroki Matsui
{"title":"Effect of rice bran extract on in vitro rumen fermentation and methane production","authors":"Jamal James D. Manlapig, Suzuna Kawakami, Masaya Matamura, Makoto Kondo, Tomomi Ban-Tokuda, Hiroki Matsui","doi":"10.1111/asj.13923","DOIUrl":null,"url":null,"abstract":"<p>This study was done to investigate which components of rice bran (RB) are involved in the inhibition of methanogenesis by fractionating the rice bran and adding it to a rumen in vitro culture system. The RB extract obtained using ethanol and water was screened in an in vitro fermentation system. The experimental treatment conditions were as follows: a control group containing a substrate without supplements; substrates with 0.06 g of RB; 0.6 mL of ethanol; 0.6 mL of distilled water (DW); 0.6 mL of ethanol-soluble fraction (ESF); 0.06 g of ethanol-insoluble rice bran (EIRB); 0.6 mL of water-soluble fraction (WSF); and 0.06 g of water-insoluble rice bran (WIRB). Based on the result of the analysis, the addition of ESF significantly decreased CH<sub>4</sub> and CH<sub>4</sub>/g dry matter digested, methanogen population (<i>p</i> < 0.05), while gas and dry matter digestibility (DMD) were comparable with the control group. Total short-chain fatty acid (SCFA), and proportion of propionate were reduced, and the proportion of butyrate was increased by the addition of ethanol and ESF (<i>p</i> < 0.05). This result suggests that the supplementation of 10% ESF can substantially reduce methane production in vitro without a negative effect on substrate digestibility.</p>","PeriodicalId":7890,"journal":{"name":"Animal Science Journal","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/asj.13923","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Science Journal","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/asj.13923","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
Abstract
This study was done to investigate which components of rice bran (RB) are involved in the inhibition of methanogenesis by fractionating the rice bran and adding it to a rumen in vitro culture system. The RB extract obtained using ethanol and water was screened in an in vitro fermentation system. The experimental treatment conditions were as follows: a control group containing a substrate without supplements; substrates with 0.06 g of RB; 0.6 mL of ethanol; 0.6 mL of distilled water (DW); 0.6 mL of ethanol-soluble fraction (ESF); 0.06 g of ethanol-insoluble rice bran (EIRB); 0.6 mL of water-soluble fraction (WSF); and 0.06 g of water-insoluble rice bran (WIRB). Based on the result of the analysis, the addition of ESF significantly decreased CH4 and CH4/g dry matter digested, methanogen population (p < 0.05), while gas and dry matter digestibility (DMD) were comparable with the control group. Total short-chain fatty acid (SCFA), and proportion of propionate were reduced, and the proportion of butyrate was increased by the addition of ethanol and ESF (p < 0.05). This result suggests that the supplementation of 10% ESF can substantially reduce methane production in vitro without a negative effect on substrate digestibility.
期刊介绍:
Animal Science Journal (a continuation of Animal Science and Technology) is the official journal of the Japanese Society of Animal Science (JSAS) and publishes Original Research Articles (full papers and rapid communications) in English in all fields of animal and poultry science: genetics and breeding, genetic engineering, reproduction, embryo manipulation, nutrition, feeds and feeding, physiology, anatomy, environment and behavior, animal products (milk, meat, eggs and their by-products) and their processing, and livestock economics. Animal Science Journal will invite Review Articles in consultations with Editors. Submission to the Journal is open to those who are interested in animal science.