The development and standardization of an enzyme-linked immunosorbent assay for the detection of antibodies to bovine herpesvirus 2

Abstract

A single dilution enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to bovine herpesvirus 2 has been developed, standardized and compared with the virus neutralization test. The results of the two tests correlated well. A positive/negative threshold was established for the ELISA. The ELISA was reproducible, sensitive, rapid and specific.