Expression and clinical significance of miR-141-5p as a biomarker in the serum of patients with early spontaneous abortion

IF 2.2 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Clinics Pub Date : 2024-01-01 DOI:10.1016/j.clinsp.2024.100327

XiaoQun Che , Xiao Wang , LiLian Wang , LiHua Xu , Lin Zou , TianZhong Ma , Bi Chen

{"title":"Expression and clinical significance of miR-141-5p as a biomarker in the serum of patients with early spontaneous abortion","authors":"XiaoQun Che , Xiao Wang , LiLian Wang , LiHua Xu , Lin Zou , TianZhong Ma , Bi Chen","doi":"10.1016/j.clinsp.2024.100327","DOIUrl":null,"url":null,"abstract":"<div><h3>Aim</h3><p>miR-141-5p expression in patients with Early Spontaneous Abortion (ESA) and its correlation with hormone levels during pregnancy were investigated.</p></div><div><h3>Methods</h3><p>A total of 70 pregnant women with ESA were selected as the research group, and 70 normal pregnant women who chose abortion for non-medical reasons were selected as the Con group. Serum β-HCG, Progesterone (P), and Estrogen (E2) were detected by enzyme-linked immunosorbent assay. Differentially expressed miRNAs were screened by miRNA microarray analysis. miR-141-5p expression was detected by RT-qPCR, and its correlation with serum β-HCG, P, and E2 levels was analyzed. The diagnostic value of miR-141-5p for ESA was evaluated by the ROC curve.</p></div><div><h3>Results</h3><p>Serum β-HCG, P, and E2 were decreased and serum miR-141-5p was increased in patients with ESA. Pearson correlation analysis showed that serum β-HCG, P, and E2 levels were negatively correlated with miR-141-5p expression levels. ROC curve showed that miR-141-5p had a diagnostic value for ESA.</p></div><div><h3>Conclusions</h3><p>miR-141-5p is related to hormone levels during pregnancy and is expected to become a new candidate diagnostic marker for ESA.</p></div>","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"79 ","pages":"Article 100327"},"PeriodicalIF":2.2000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1807593224000048/pdfft?md5=1d3211418aaf461c0791cd4e5c6a1407&pid=1-s2.0-S1807593224000048-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinics","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1807593224000048","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}

引用次数: 0

Abstract

Aim

miR-141-5p expression in patients with Early Spontaneous Abortion (ESA) and its correlation with hormone levels during pregnancy were investigated.

Methods

A total of 70 pregnant women with ESA were selected as the research group, and 70 normal pregnant women who chose abortion for non-medical reasons were selected as the Con group. Serum β-HCG, Progesterone (P), and Estrogen (E2) were detected by enzyme-linked immunosorbent assay. Differentially expressed miRNAs were screened by miRNA microarray analysis. miR-141-5p expression was detected by RT-qPCR, and its correlation with serum β-HCG, P, and E2 levels was analyzed. The diagnostic value of miR-141-5p for ESA was evaluated by the ROC curve.

Results

Serum β-HCG, P, and E2 were decreased and serum miR-141-5p was increased in patients with ESA. Pearson correlation analysis showed that serum β-HCG, P, and E2 levels were negatively correlated with miR-141-5p expression levels. ROC curve showed that miR-141-5p had a diagnostic value for ESA.

Conclusions

miR-141-5p is related to hormone levels during pregnancy and is expected to become a new candidate diagnostic marker for ESA.

查看原文本刊更多论文

早期自然流产患者血清中作为生物标志物的 miR-141-5p 的表达及其临床意义。

目的：研究早期自然流产（ESA）患者体内miR-141-5p的表达及其与孕期激素水平的相关性：方法：选取70例ESA孕妇为研究组，70例非医学原因选择流产的正常孕妇为对照组。采用酶联免疫吸附法检测血清β-HCG、孕酮（P）和雌激素（E2）。通过 RT-qPCR 检测了 miR-141-5p 的表达，并分析了其与血清 β-HCG、P 和 E2 水平的相关性。用ROC曲线评估了miR-141-5p对ESA的诊断价值：结果：ESA患者的血清β-HCG、P和E2水平降低，血清miR-141-5p水平升高。皮尔逊相关分析显示，血清β-HCG、P和E2水平与miR-141-5p表达水平呈负相关。结论：miR-141-5p与妊娠期激素水平有关，有望成为ESA新的候选诊断标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Clinics 医学-医学：内科

CiteScore

4.10

自引率

3.70%

发文量

129

审稿时长

52 days

期刊介绍： CLINICS is an electronic journal that publishes peer-reviewed articles in continuous flow, of interest to clinicians and researchers in the medical sciences. CLINICS complies with the policies of funding agencies which request or require deposition of the published articles that they fund into publicly available databases. CLINICS supports the position of the International Committee of Medical Journal Editors (ICMJE) on trial registration.