Propofol Modulates Gallbladder Cancer Progression via miR-4430/SLC19A3 Axis.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-01

Hui Zhang, Bei Zhang

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引用次数: 0

Abstract

Background/aim: Propofol is a common anesthetic used during surgery. MircoRNA (miRNA), especially miR-4430, is currently a research hotspot in GBC. This study investigates whether propofol regulates GBC through the miR-4430/SLC19A3 axis.

Materials and methods: NOZ and SGC996 cells, human gallbladder cancer cell lines, were obtained from BLUEFBIO (Shanghai, China). The cells were treated with propofol, and the experimental setup included a control group. qRT-PCR was used to measure the content of miR-4430 and solute carrier family 19 member 3 (SLC19A3). The cell viability, migration, and apoptosis were analyzed by colony formation assay, wound healing assay, and flow cytometry, respectively. Western blot tested the levels of SLC91A3, cyclin D-dependent kinases 6 (CDK6), and Bax proteins. Mechanically, dual-luciferase reporter assay notarized the link of miR-4430 with SLC19A3.

Results: Propofol significantly repressed the proliferation of GBC cells, as indicated by a pronounced reduction in colony formation in both NOZ and SGC996 cells (P = .025, 95% CI:1.2, 2.4). The inhibitory effect of propofol was reversed by the enhanced expression of miR-4430 in GBC cells. Furthermore, SLC19A3 was identified as a direct target of miR-4430. Silencing SLC19A3 reversed the altered cell behavior observed in propofol-treated GBC cells (P = .019, 95% CI:1.6, 3.5). Additionally, overexpression of SLC19A3 significantly attenuated both cell proliferation and migration influenced by miR-4430 in propofol-treated GBC cells (P = .034, 95% CI:1.4, 2.8).

Conclusion: Propofol changed the cell behaviors of GBC by modulating the miR-4430/SLC19A3 axis.

本刊更多论文

丙泊酚通过 miR-4430/SLC19A3 轴调节胆囊癌进展

背景/目的：丙泊酚是手术中常用的麻醉剂。MircoRNA（miRNA），尤其是 miR-4430，是目前 GBC 的研究热点。本研究探讨了异丙酚是否通过 miR-4430/SLC19A3 轴调控 GBC：人胆囊癌细胞株 NOZ 和 SGC996 细胞来自博路生物（上海）有限公司。用 qRT-PCR 检测 miR-4430 和溶质运载家族 19 成员 3（SLC19A3）的含量。细胞活力、迁移和凋亡分别通过集落形成试验、伤口愈合试验和流式细胞术进行分析。Western blot 检测了 SLC91A3、细胞周期蛋白 D 依赖性激酶 6（CDK6）和 Bax 蛋白的水平。双荧光素酶报告实验证实了 miR-4430 与 SLC19A3 的关系：结果：异丙酚明显抑制了 GBC 细胞的增殖，NOZ 和 SGC996 细胞的集落形成明显减少（P = .025，95% CI：1.2, 2.4）。异丙酚的抑制作用因 miR-4430 在 GBC 细胞中的表达增强而逆转。此外，SLC19A3 被确定为 miR-4430 的直接靶标。沉默 SLC19A3 逆转了异丙酚处理的 GBC 细胞中观察到的细胞行为改变（P = .019，95% CI：1.6, 3.5）。此外，在异丙酚处理的 GBC 细胞中，过表达 SLC19A3 能显著减轻 miR-4430 对细胞增殖和迁移的影响（P = .034，95% CI：1.4, 2.8）：结论：异丙酚通过调节miR-4430/SLC19A3轴改变了GBC细胞的行为。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.