Biological and physical properties of calcium hydroxide-based pulp-capping materials and their modifications.

IF 1.9 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Journal of oral science Pub Date : 2024-04-16 Epub Date: 2024-02-08 DOI:10.2334/josnusd.23-0288
Dilek Akin, Cigdem Atalayin Ozkaya, Guliz Armagan, Dervis Birim, Mustafa Ates, Huseyin Tezel
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引用次数: 0

Abstract

Purpose: To evaluate the biological and physical properties of calcium hydroxide-containing pulp-capping materials and their modifications with different solutions and antioxidant Resveratrol (RES) addition.

Methods: Calcium hydroxide+distilled-water:C, calcium hydroxide+saline:S, calcium hydroxide+synthetic tissue fluid:STF, Dycal:D, calcium hydroxide+distilled-water+RES:C+RES, calcium hydroxide+saline+RES:S+RES, calcium hydroxide+synthetic tissue fluid+RES:STF+RES, Dycal+RES:D+RES were tested. Cytotoxicity was determined by WST-1. Antibacterial-activity was evaluated by agar-diffusion. The water-absorption and solubility were examined for ISO-6876 and ISO-3107. The color-change was evaluated by spectrophotometer. Radiopacity was evaluated for ISO-6876 and ISO-9917. The normal distribution and homogeneity were determined and comparisons were made with appropriate analysis and post hoc tests (P < 0.05).

Results: The highest cell-viability was determined in the C+RES and the lowest was in D and D+RES after 24 h (P < 0.0001). RES-addition increased cell-viability and the highest rate was detected in C+RES, S+RES and STF+RES after 48 h (P < 0.0001). A limited inhibition-zone against Streptococcus mutans was detected in D and D+RES. RES-addition did not change the water-absorption in S and STF or the solubility in S group.

Conclusion: RES-addition may be used to increase the biocompatibility of calcium hydroxide without any adverse effect on physical properties. Saline may be the first choice as a mixing solution.

基于氢氧化钙的纸浆盖材料及其改性的生物和物理特性。
目的:评估含氢氧化钙的牙髓覆盖材料的生物和物理特性,以及不同溶液和抗氧化剂白藜芦醇(RES)对其的改性:氢氧化钙+蒸馏水:C、氢氧化钙+生理盐水:S、氢氧化钙+合成组织液:STF、Dycal:D、氢氧化钙+蒸馏水+RES:C+RES、氢氧化钙+生理盐水+RES:S+RES、氢氧化钙+合成组织液+RES:STF+RES、Dycal+RES:D+RES。细胞毒性由 WST-1 测定。抗菌活性通过琼脂扩散法进行评估。ISO-6876 和 ISO-3107 检测了吸水性和溶解性。用分光光度计评估颜色变化。对 ISO-6876 和 ISO-9917 进行了不透射线性评估。测定了正态分布和均匀性,并通过适当的分析和事后检验(P < 0.05)进行了比较:结果:24 小时后,C+RES 的细胞存活率最高,D 和 D+RES 的细胞存活率最低(P < 0.0001)。添加 RES 可提高细胞活力,48 小时后,C+RES、S+RES 和 STF+RES 的细胞活力最高(P < 0.0001)。在 D 和 D+RES 中检测到了对变异链球菌的有限抑制区。添加 RES 不会改变 S 组和 STF 组的吸水性或 S 组的溶解性:结论:添加 RES 可用于提高氢氧化钙的生物相容性,而不会对物理性质产生任何不利影响。生理盐水可作为混合溶液的首选。
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来源期刊
Journal of oral science
Journal of oral science DENTISTRY, ORAL SURGERY & MEDICINE-MATERIALS SCIENCE, BIOMATERIALS
CiteScore
3.80
自引率
0.00%
发文量
58
期刊介绍: Information not localized
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