Gleyce Ha de Souza, Marcia Sm Vaz, Joyce A Dos Santos Radai, Thiago L Fraga, Luana Rossato, Simone Simionatto
{"title":"Synergistic interaction of polymyxin B with carvacrol: antimicrobial strategy against polymyxin-resistant <i>Klebsiella pneumoniae</i>.","authors":"Gleyce Ha de Souza, Marcia Sm Vaz, Joyce A Dos Santos Radai, Thiago L Fraga, Luana Rossato, Simone Simionatto","doi":"10.2217/fmb-2023-0070","DOIUrl":null,"url":null,"abstract":"<p><p><b>Objective:</b> The antimicrobial activities of the synergistic combination of carvacrol and polymyxin B against polymyxin-resistant <i>Klebsiella pneumoniae</i> were evaluated. <b>Methods:</b> The methods employed checkerboard assays to investigate synergism, biofilm inhibition assessment and membrane integrity assay. In addition, the study included <i>in vivo</i> evaluation using a mouse infection model. <b>Results:</b> The checkerboard method evaluated 48 combinations, with 23 indicating synergistic action. Among these, carvacrol 10 mg/kg plus polymyxin B 2 mg/kg exhibited <i>in vivo</i> antimicrobial activity in a mouse model of infection, resulting in increased survival and a significant decrease in bacterial load in the blood. <b>Conclusion:</b> Polymyxin in synergy with carvacrol represents a promising alternative to be explored in the development of new antimicrobials.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.2217/fmb-2023-0070","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/8 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: The antimicrobial activities of the synergistic combination of carvacrol and polymyxin B against polymyxin-resistant Klebsiella pneumoniae were evaluated. Methods: The methods employed checkerboard assays to investigate synergism, biofilm inhibition assessment and membrane integrity assay. In addition, the study included in vivo evaluation using a mouse infection model. Results: The checkerboard method evaluated 48 combinations, with 23 indicating synergistic action. Among these, carvacrol 10 mg/kg plus polymyxin B 2 mg/kg exhibited in vivo antimicrobial activity in a mouse model of infection, resulting in increased survival and a significant decrease in bacterial load in the blood. Conclusion: Polymyxin in synergy with carvacrol represents a promising alternative to be explored in the development of new antimicrobials.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
