Genotypic and Allelic Prevalence of the TGF- Β1 +869 C/T SNP and Their Relationship to Seminogram in Infertile Males.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ahmed Abdulqader Al-Naqshbandi, Suhaila Nafee Darogha, Kalthum Asaaf Maulood
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引用次数: 0

Abstract

Background: The influence of cytokine in the reproductive system is becoming increasingly important. The polymorphisms of the transforming growth factor-β1 (TGF-β1) gene are involved in male infertility. This study aimed to demonstrate the association between TGF-β1 and infertility and to investigate its impact on semen quality.

Methods: In this case-control study, serum TGF-β1 concentration was measured in 144 patients diagnosed with infertility and 40 fertile males by enzyme-linked immunosorbent assay (ELISA). The tetra-amplification refractory mutation system-PCR (T-ARMS-PCR) analysis was performed to detect the genotyping of the TGF-β1 (+869 C/T) (rs1800470) SNPs gene.

Results: Serum concentration of TGF-β1 was less in infertile males compared to fertile ones. The detected and more effective genotypes and alleles of TGF-β1 gene polymorphic on male infertility were, in normozoospermic group, CT genotype, probability (p)= 0.45, relative risk (RR)= 1.56, confidence intervals (CI): 0.58-4.22, and T allele (p= 0.46, RR= 1.32, CI: 0.65-2.69), in oligozoospermic and azoospermic groups, CC genotype (p= 0.32, RR= 1.58, CI: 0.73-3.41), (p= 0.013, RR= 3.50, CI: 1.40-8.73), and allele C (p= 0.44, RR= 1.32, CI: 0.73-2.38), (p= 0.06, RR= 2.14, CI: 1.02-4.50), respectively. The recessive model (TT+CT) showed increased risk among normozoospermic group (p=0.44, RR=1.67, CI:0.60-4.62). The serum concentration of TGF-β1 with CT and TT genotypes was less than that of CC genotype. TGF-β1 C/T genotype correlated with low sperm number, high immotile sperm, and high abnormal sperm morphology.

Conclusions: Our study revealed that the TGF-β1(rs1800470) gene polymorphisms are associated negatively with semen quality.

不育男性中 TGF- Β1 +869 C/T SNP 的基因型和等位基因流行率及其与精液图的关系。
背景:细胞因子对生殖系统的影响正变得越来越重要。转化生长因子-β1(TGF-β1)基因的多态性与男性不育有关。本研究旨在证明 TGF-β1 与不育之间的关联,并探讨其对精液质量的影响:在这项病例对照研究中,采用酶联免疫吸附试验(ELISA)测定了 144 名确诊不育症患者和 40 名育龄男性的血清 TGF-β1 浓度。采用四扩增难治性突变系统-PCR(T-ARMS-PCR)分析法检测 TGF-β1 (+869 C/T) (rs1800470) SNPs 基因的基因分型:结果:与可育男性相比,不育男性血清中的 TGF-β1 浓度较低。检测到的对男性不育更有效的 TGF-β1 基因多态性基因型和等位基因是:在正常无精子组中,CT 基因型,概率(p)= 0.45,相对风险(RR)= 1.56,置信区间(CI):0.58-4.22,T 等位基因(p= 0.46,RR= 1.32,置信区间(CI):0.65-2.69),在少精子症组和无精子症组,CC 基因型(p= 0.32,RR= 1.58,CI:0.73-3.41)、(p= 0.013,RR= 3.50,CI:1.40-8.73),以及等位基因 C(p= 0.44,RR= 1.32,CI:0.73-2.38)、(p= 0.06,RR= 2.14,CI:1.02-4.50)。隐性模型(TT+CT)显示正常无精子组的风险增加(P=0.44,RR=1.67,CI:0.60-4.62)。CT 和 TT 基因型血清中的 TGF-β1 浓度低于 CC 基因型。TGF-β1 C/T基因型与精子数量少、无活力精子多和精子形态异常有关:我们的研究表明,TGF-β1(rs1800470)基因多态性与精液质量呈负相关。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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