Development of Electrochemical Immunosensors for Early Diagnosis of Polycystic Ovary Syndrome (PCOS), and Their Potential Mobile Phone Application

IF 2.8 3区化学 Q2 CHEMISTRY, APPLIED

Topics in Catalysis Pub Date : 2024-02-03 DOI:10.1007/s11244-023-01899-0

Muhammad Ali Yousif Al-Janabi, Ramazan Bayat, Muhammed Bekmezci, Tiri Rima Nour Elhouda, Fatih Sen, Afsaneh Kaffash, Mehdi Baghayeri, Hassan Rokni, Fatemeh Karimi

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Abstract

An effective way of monitoring the biomarker is with electrochemical sensor studies. In this work, it was formed using electrochemical deposition of high conductivity gold nanoparticles (AuNPs) and an amine-functional reduced graphene oxide (NH₂-RGO) nanocomposite. This technique not only reduced HAuCl₄ and graphene oxide in situ but also improved the electrocatalytic performance. XRD, SEM, and FTIR analytical methods confirmed the size and structure of the AuNPs/NH₂-RGO nanoparticle. As a result of XRD analysis, AuNPs/NH2-RGO crystal structure was formed. In addition, antibodies (Ab) were immobilized on the modified electrode surface using a self-assembled monolayer. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods were used to evaluate the interaction of Ab with antigen. SHBG detection had a dynamic linear range of 0.6–12 nmol/L and a limit of detection (LOD) of 0.0043 µM in enhanced sensor applications. The AuNPs/NH₂-RGO based sensor has the potential to be a valuable tool in clinical diagnostic applications in the future.

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开发用于多囊卵巢综合征 (PCOS) 早期诊断的电化学免疫传感器及其潜在的手机应用

电化学传感器研究是监测生物标志物的有效方法。在这项工作中，利用电化学沉积高电导率金纳米粒子（AuNPs）和胺功能还原氧化石墨烯（NH2-RGO）纳米复合材料形成了这种传感器。这种技术不仅能原位还原 HAuCl4 和氧化石墨烯，还能提高电催化性能。XRD、SEM 和 FTIR 分析方法证实了 AuNPs/NH2-RGO 纳米粒子的尺寸和结构。XRD 分析结果表明，AuNPs/NH2-RGO 形成了晶体结构。此外，还利用自组装单层法将抗体（Ab）固定在改性电极表面。采用循环伏安法（CV）和差分脉冲伏安法（DPV）评估抗体与抗原的相互作用。在增强型传感器应用中，SHBG 检测的动态线性范围为 0.6-12 nmol/L，检测限 (LOD) 为 0.0043 µM。基于 AuNPs/NH2-RGO 的传感器有望成为未来临床诊断应用的重要工具。

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来源期刊

Topics in Catalysis 化学-物理化学

CiteScore

5.70

自引率

5.60%

发文量

197

审稿时长

2 months

期刊介绍： Topics in Catalysis publishes topical collections in all fields of catalysis which are composed only of invited articles from leading authors. The journal documents today’s emerging and critical trends in all branches of catalysis. Each themed issue is organized by renowned Guest Editors in collaboration with the Editors-in-Chief. Proposals for new topics are welcome and should be submitted directly to the Editors-in-Chief. The publication of individual uninvited original research articles can be sent to our sister journal Catalysis Letters. This journal aims for rapid publication of high-impact original research articles in all fields of both applied and theoretical catalysis, including heterogeneous, homogeneous and biocatalysis.