miR-26b-5p Affects the Progression of Acute Myeloid Leukemia by Regulating the USP48-Mediated Wnt/β-Catenin Pathway

IF 1.5 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-01-01 DOI:10.1615/critreveukaryotgeneexpr.2024049380

Yu Xie, Lin Tan, Kun Wu, Deyun Li, Chengping Li

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引用次数: 0

Abstract

Acute myeloid leukemia (AML) is a highly heterogeneous disease. Exploring the pathogenesis of AML is still an important topic in the treatment of AML. The expression levels of miR-26b-5p and USP48 were measured by qRT-PCR. The expression levels of related proteins were detected by Western blot. Cell proliferation and apoptosis were detected by CCK-8 and flow cytometry, respectively. Coimmunoprecipitation was used to examine the interaction between USP48 and Wnt5a. Bioinformatics analysis showed that high levels of miR-26b-5p and low levels of USP48 were associated with poor prognosis in AML. miR-26b-5p can negatively regulate the expression of USP48. Downregulation of miR-26b-5p inhibited EMT, cell viability and proliferation of AML cells and accelerated apoptosis. Furthermore, the influence of miR-26b-5p inhibition and USP48 knockdown on AML progression could be reversed by a Wnt/β-catenin signaling pathway inhibitor. This study revealed that miR-26b-5p regulates AML progression, possibly by targeting the USP48-mediated Wnt/β-catenin molecular axis to affect AML cell biological behavior.

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miR-26b-5p 通过调控 USP48 介导的 Wnt/β-Catenin 通路影响急性髓性白血病的进展

急性髓性白血病（AML）是一种高度异质性疾病。探索急性髓性白血病的发病机制仍是治疗急性髓性白血病的重要课题。研究人员通过 qRT-PCR 法检测了 miR-26b-5p 和 USP48 的表达水平。通过 Western 印迹检测相关蛋白的表达水平。细胞增殖和凋亡分别通过 CCK-8 和流式细胞术检测。共免共沉淀用于检测 USP48 和 Wnt5a 之间的相互作用。生物信息学分析表明，高水平的miR-26b-5p和低水平的USP48与急性髓细胞性白血病的不良预后有关。下调 miR-26b-5p 可抑制 AML 细胞的 EMT、细胞活力和增殖，并加速细胞凋亡。此外，Wnt/β-catenin 信号通路抑制剂可逆转 miR-26b-5p 抑制和 USP48 敲除对 AML 进展的影响。这项研究揭示了miR-26b-5p可能通过靶向USP48介导的Wnt/β-catenin分子轴来影响AML细胞的生物学行为，从而调控AML的进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.