Jin-Ho Kim, Hae Lim Kim, Sea-Hee Han, Hwa Lee, Jong-Eun Han, Tea-Su Kim, Su Hui Seong, Bo-Ram Kim, Ha-Nul Lee, Chan Seo, Jung Eun Kim, Sua Im, Ji Min Jung, Kyung-Min Choi, Jin-Woo Jeong
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引用次数: 0
Abstract
Glochidion chodoense C. S. Lee & H. T. Im is an endangered plant found in Korea. Its conservation and potential industrial utilization require the development of suitable genetic resource propagation approaches. We here present a protocol for the efficient propagation of G. chodoense leaf segments by callus induction. We assessed various induction media containing different concentrations of plant growth regulators, as well as antioxidants to inhibit callus browning. The use of MS medium with 2,4-Di-chlorophenoxy-acetic acid (2,4-D) (1 mg/L) and BA (0.5 mg/L) for callus induction and incorporating ascorbic acid (10 mg/L) to mitigate browning was found to offer a promising approach for the mass production of G. chodoense. This protocol has significant potential for the conservation and industrial utilization of this endangered species.
一种濒危韩国本地植物 Glochidion chodoense 的体外胼胝体诱导和药用生长 C. S. Lee & H. T. Im.
Glochidion chodoense C. S. Lee & H. T. Im 是韩国的一种濒危植物。要保护这种植物并将其用于潜在的工业用途,就需要开发合适的遗传资源繁殖方法。我们在此介绍一种通过胼胝体诱导高效繁殖 G. chodoense 叶片的方法。我们评估了含有不同浓度植物生长调节剂和抗氧化剂的各种诱导培养基,以抑制胼胝体褐变。结果发现,使用含有 2,4-D (2,4-D)(1 毫克/升)和 BA(0.5 毫克/升)的 MS 培养基进行胼胝体诱导,并加入抗坏血酸(10 毫克/升)以减轻褐变,为 G. chodoense 的大规模生产提供了一种可行的方法。该方案在这一濒危物种的保护和工业利用方面具有巨大潜力。
RhizosphereAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
5.70
自引率
8.10%
发文量
155
审稿时长
29 days
期刊介绍:
Rhizosphere aims to advance the frontier of our understanding of plant-soil interactions. Rhizosphere is a multidisciplinary journal that publishes research on the interactions between plant roots, soil organisms, nutrients, and water. Except carbon fixation by photosynthesis, plants obtain all other elements primarily from soil through roots.
We are beginning to understand how communications at the rhizosphere, with soil organisms and other plant species, affect root exudates and nutrient uptake. This rapidly evolving subject utilizes molecular biology and genomic tools, food web or community structure manipulations, high performance liquid chromatography, isotopic analysis, diverse spectroscopic analytics, tomography and other microscopy, complex statistical and modeling tools.