{"title":"A new universal detection system for high-performance thin-layer chromatography plates using chemiluminescence","authors":"Bernd Spangenberg","doi":"10.1007/s00764-024-00286-z","DOIUrl":null,"url":null,"abstract":"<p>High-performance thin-layer chromatography (HPTLC) is a cost-effective method for the separation of small molecules, such as active pharmaceutically ingredients. The method can be used without sophisticated equipment. For example, the detection and quantification of colored compounds on an HPTLC plate can be performed with a CCD camera. For noncolored compounds, a new dipping solution for the generation of chemiluminescence on an HPTLC plates is presented. In combination with a charge-coupled diode (CCD) camera, this can be used as a universal detection system. The efficiency of this new staining reagent is demonstrated by the detection of trimethoprim and sulfamethoxazole from a fixed-dose combination in a ratio of 1:5.</p>","PeriodicalId":17616,"journal":{"name":"Jpc-journal of Planar Chromatography-modern Tlc","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jpc-journal of Planar Chromatography-modern Tlc","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s00764-024-00286-z","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
High-performance thin-layer chromatography (HPTLC) is a cost-effective method for the separation of small molecules, such as active pharmaceutically ingredients. The method can be used without sophisticated equipment. For example, the detection and quantification of colored compounds on an HPTLC plate can be performed with a CCD camera. For noncolored compounds, a new dipping solution for the generation of chemiluminescence on an HPTLC plates is presented. In combination with a charge-coupled diode (CCD) camera, this can be used as a universal detection system. The efficiency of this new staining reagent is demonstrated by the detection of trimethoprim and sulfamethoxazole from a fixed-dose combination in a ratio of 1:5.
期刊介绍：
JPC - Journal of Planar Chromatography - Modern TLC is an international journal devoted exclusively to the publication of research papers on analytical and preparative planar chromatography. The journal covers all fields of planar chromatography, on all kinds of stationary phase (paper, layer, gel) and with various modes of migration of the mobile phase (capillary action or forced flow).
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