Increased expression of keratin 17 in oral lichen planus and its correlation with disease severity

IF 3.4 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Xinyi Han , Ran Zhao , Qianqian Zhang, Xuemin Shen, Kai Sun
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引用次数: 0

Abstract

Background/purpose

Oral lichen planus (OLP) is a chronic inflammatory disease with unknown mechanisms of pathogenesis. Keratin 17 (KRT17) is a protein that regulates numerous cellular processes. This study aimed to explore the expression of KRT17 in OLP and its correlation with the severity of OLP.

Materials and methods

RNA sequencing using epithelium from 5 OLP patients and 5 health control (HC) was performed, followed by functional analysis. The validation cohort of 20 OLP and 20 HC tissues were used to investigate positive area value of KRT17 by immunohistochemical analysis. Reticular, erosive and ulcerative (REU) scores were used for measuring the severity of OLP.

Results

A total of 15493 genes were detected, of which 1492 genes were significantly up-regulated in OLP and 622 were down-regulated. The mRNA expression of KRT17 was elevated by 13.09-fold in OLP compared to that in HC. Pathway analysis demonstrated high KRT17 expression was associated with multiple biological processes. The median of percentage of KRT17 positive area value was 19.30 % in OLP and 0.01 % in HC (P < 0.001). Percentage of KRT17 positive area value was higher in erosive OLP patients (27.25 %) compared to that in non-erosive patients (15.02 %, P = 0.006). REU scores were positively correlated with percentage of KRT17 positive area value (r = 0.628, P = 0.003).

Conclusion

The mRNA expression of KRT17 was elevated in OLP tissues compared to that in HC. KRT17 was positively correlated with the severity of OLP, indicating KRT17 might play a vital role in the pathogenesis of OLP.

口腔扁平苔藓中角蛋白 17 的表达增加及其与疾病严重程度的关系
背景/目的口腔扁平苔藓(OLP)是一种慢性炎症性疾病,发病机制不明。角蛋白17(KRT17)是一种调节多种细胞过程的蛋白质。本研究旨在探讨KRT17在OLP中的表达及其与OLP严重程度的相关性。材料与方法利用5名OLP患者和5名健康对照者(HC)的上皮细胞进行RNA测序,然后进行功能分析。对 20 例 OLP 和 20 例 HC 组织进行验证,通过免疫组化分析研究 KRT17 的阳性面积值。结果 共检测到 15493 个基因,其中 1492 个基因在 OLP 中显著上调,622 个基因下调。与HC相比,KRT17的mRNA表达在OLP中升高了13.09倍。通路分析表明,KRT17的高表达与多个生物学过程相关。KRT17阳性面积百分比的中位数在OLP中为19.30%,在HC中为0.01%(P < 0.001)。侵蚀性OLP患者的KRT17阳性面积值百分比(27.25%)高于非侵蚀性患者(15.02%,P = 0.006)。REU评分与KRT17阳性面积百分比呈正相关(r = 0.628, P = 0.003)。KRT17与OLP的严重程度呈正相关,表明KRT17可能在OLP的发病机制中发挥重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Dental Sciences
Journal of Dental Sciences 医学-牙科与口腔外科
CiteScore
5.10
自引率
14.30%
发文量
348
审稿时长
6 days
期刊介绍: he Journal of Dental Sciences (JDS), published quarterly, is the official and open access publication of the Association for Dental Sciences of the Republic of China (ADS-ROC). The precedent journal of the JDS is the Chinese Dental Journal (CDJ) which had already been covered by MEDLINE in 1988. As the CDJ continued to prove its importance in the region, the ADS-ROC decided to move to the international community by publishing an English journal. Hence, the birth of the JDS in 2006. The JDS is indexed in the SCI Expanded since 2008. It is also indexed in Scopus, and EMCare, ScienceDirect, SIIC Data Bases. The topics covered by the JDS include all fields of basic and clinical dentistry. Some manuscripts focusing on the study of certain endemic diseases such as dental caries and periodontal diseases in particular regions of any country as well as oral pre-cancers, oral cancers, and oral submucous fibrosis related to betel nut chewing habit are also considered for publication. Besides, the JDS also publishes articles about the efficacy of a new treatment modality on oral verrucous hyperplasia or early oral squamous cell carcinoma.
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