H3K27ac modification and transcription characteristics of adipose and muscle tissues in Chuxiang Black pig

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-01-31 DOI:10.1111/age.13400

Renzhuo Kuang, Zhixiang Xu, Honghong Zhou, Zhao Zhang, Hao Peng, Daoyuan Wang, Xuewen Xu, Shuhong Zhao, Yunxia Zhao, Mengjin Zhu

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Abstract

The establishment of high-quality pork breeds for improving meat quality in the pig industry is needed. The Chuxiang Black (CX) pig is a new breed developed from Chinese local pigs and Western lean pigs that has a high proportion of lean meat and excellent meat quality. However, the characteristics of cis-regulatory elements in CX pigs are still unknown. In this study, cis-regulatory elements of muscle and adipose tissues in CX pigs were investigated using ChIP-seq and RNA sequencing. Compared with the reported cis-regulatory elements of muscle and adipose tissues, 1768 and 1012 highly activated enhancers and 433 and 275 highly activated promoters in CX muscle and adipose tissues were identified, respectively. Motif analysis showed that transcription factors, such as MEF2A and MEF2C, were core regulators of highly activated enhancers and promoters in muscle. Similarly, the transcription factors JUNB and CUX1 were identified as essential for highly activated enhancers and promoters in CX adipose tissue. These results enrich the resources for the analysis of cis-regulatory elements in the pig genome and provide new basic data for further meat quality improvement through breeding in pigs.

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竹乡黑猪脂肪和肌肉组织的 H3K27ac 修饰及转录特征

养猪业需要建立优质猪肉品种以提高肉质。楚香黑（CX）猪是由中国地方猪和西方瘦肉型猪培育而成的新品种，瘦肉率高，肉质优良。然而，CX 猪的顺式调控因子的特征仍然未知。本研究利用 ChIP-seq 和 RNA 测序技术研究了 CX 猪肌肉和脂肪组织中的顺式调控因子。与已报道的肌肉和脂肪组织顺式调控元件相比，在 CX 肌肉和脂肪组织中分别发现了 1768 个和 1012 个高度激活的增强子以及 433 个和 275 个高度激活的启动子。动因分析表明，MEF2A 和 MEF2C 等转录因子是肌肉中高度激活的增强子和启动子的核心调控因子。同样，转录因子JUNB和CUX1也被鉴定为CX脂肪组织中高度激活的增强子和启动子所必需的转录因子。这些结果丰富了分析猪基因组顺式调控元件的资源，为通过育种进一步提高猪肉品质提供了新的基础数据。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.