{"title":"Induction of the Cholinergic Phenotype in Mouse Neuroblastoma Cells Using Nerve Growth Factor","authors":"","doi":"10.1134/s181971242304013x","DOIUrl":null,"url":null,"abstract":"<p><strong>Abstract</strong>—Nerve growth factor (NGF) is a factor which determines neuronal differentiation. NGF plays an important role in growth and differentiation of sensory and sympathetic neurons in the peripheral nervous system. In the mature brain, NGF is involved in the maintenance of the cholinergic neuronal phenotype. Here, we studied a possibility to induce the cholinergic phenotype in mouse neuroblastoma cells, which are often used to model various physiological and pathological processes occurring in the nervous system. Cells of NB41A3 and Neuro2a neuroblastoma lines are most frequently used to study the properties of cholinergic neurons. In the cells of these lines, the expression of TrkA and p75NGFR receptors, which is specific for the forebrain cholinergic nuclei, was revealed. Differentiation of the cells was induced by application of NGF or 8-Br-cAMP. NGF did not induce neuronal differentiation. Moreover, we did not find any changes in the content of choline acetyltransferase and vesicular acetylcholine transporter mRNA and protein, which were used as markers of the cholinergic phenotype. Thus, NB41A3 and Neuro2a cell lines cannot be advised as a model of cholinergic neurons in vitro because they do not differentiate and/or exhibit signs of the cholinergic phenotype in response to NGF stimulation.</p>","PeriodicalId":19119,"journal":{"name":"Neurochemical Journal","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurochemical Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1134/s181971242304013x","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract—Nerve growth factor (NGF) is a factor which determines neuronal differentiation. NGF plays an important role in growth and differentiation of sensory and sympathetic neurons in the peripheral nervous system. In the mature brain, NGF is involved in the maintenance of the cholinergic neuronal phenotype. Here, we studied a possibility to induce the cholinergic phenotype in mouse neuroblastoma cells, which are often used to model various physiological and pathological processes occurring in the nervous system. Cells of NB41A3 and Neuro2a neuroblastoma lines are most frequently used to study the properties of cholinergic neurons. In the cells of these lines, the expression of TrkA and p75NGFR receptors, which is specific for the forebrain cholinergic nuclei, was revealed. Differentiation of the cells was induced by application of NGF or 8-Br-cAMP. NGF did not induce neuronal differentiation. Moreover, we did not find any changes in the content of choline acetyltransferase and vesicular acetylcholine transporter mRNA and protein, which were used as markers of the cholinergic phenotype. Thus, NB41A3 and Neuro2a cell lines cannot be advised as a model of cholinergic neurons in vitro because they do not differentiate and/or exhibit signs of the cholinergic phenotype in response to NGF stimulation.
期刊介绍：
Neurochemical Journal (Neirokhimiya) provides a source for the communication of the latest findings in all areas of contemporary neurochemistry and other fields of relevance (including molecular biology, biochemistry, physiology, neuroimmunology, pharmacology) in an afford to expand our understanding of the functions of the nervous system. The journal presents papers on functional neurochemistry, nervous system receptors, neurotransmitters, myelin, chromaffin granules and other components of the nervous system, as well as neurophysiological and clinical aspects, behavioral reactions, etc. Relevant topics include structure and function of the nervous system proteins, neuropeptides, nucleic acids, nucleotides, lipids, and other biologically active components.
The journal is devoted to the rapid publication of regular papers containing the results of original research, reviews highlighting major developments in neurochemistry, short communications, new experimental studies that use neurochemical methodology, descriptions of new methods of value for neurochemistry, theoretical material suggesting novel principles and approaches to neurochemical problems, presentations of new hypotheses and significant findings, discussions, chronicles of congresses, meetings, and conferences with short presentations of the most sensational and timely reports, information on the activity of the Russian and International Neurochemical Societies, as well as advertisements of reagents and equipment.
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