Aatifa Rasool, K. M. Bhat, M. A. Mir, A. S. Sundouri, Salha Mesfer ALshamrani, Abeer S. Aloufi, Diaa Abd El Moneim, Sheikh Mansoor, Yong Suk Chung
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引用次数: 0
Abstract
In recent years, there has been significant progress in enhancing the genetic foundation underlying important agricultural traits such as resistance to scab and the development of a columnar growth habit. V. inaequalis is a hemibiotrophic fungus widely distributed in temperate regions where apples are grown on commercial scale. The present investigation was undertaken to identify Vf gene and Co gene, which, respectively, confer resistance against apple scab disease and columnar phenotype in apple cultivar ‘Rosalie’ and introgression of both the genes in commercially important cultivar ‘Fuji’. Polymorphism survey was carried out between the two parents using 22 simple sequence repeat (SSR) and sequence-characterized amplified region (SCAR) markers. The observations revealed that almost 50% hybrids fall in resistant category and 50% in susceptible category. The results of marker-assisted screening confirmed 38 F1s carrying resistance gene for scab while the remaining 32 F1 plants were found to be lacking the gene. The 38 genotypically scab-resistant hybrids were selected for further characterization as columnar and non-columnar plants. Based on the selection criteria, 21 individuals were categorized as columnar and the remaining 17 were categorized as non-columnar. The phenotypic screening was followed by screening of F1 s using molecular markers for Co gene. The amplification of Co-specific markers yielded columnar-specific fragments in the population and fitted the expected 1:1 Mendelian ratio. 18 scab-resistant F1 hybrids were found to carry Co gene and the remaining 20 did not possess the gene for columnar growth habit. Gene-specific primers identified in the present study can be directly used for screening large apple germplasm in a short period of time for developing resistant varieties against apple scab as well as varieties with columnar growth habit. Hybrids with verified scab resistance and columnar growth can be swiftly utilized as scab-resistant columnar cultivars.
近年来，在提高抗疮痂病和柱状生长习性等重要农业性状的遗传基础方面取得了重大进展。V. inaequalis 是一种半生营养真菌，广泛分布于苹果商业化种植的温带地区。本研究旨在鉴定 Vf 基因和 Co 基因，它们分别赋予苹果栽培品种 "Rosalie "对苹果疮痂病和柱状表型的抗性，并将这两种基因导入商业上重要的栽培品种 "Fuji"。利用 22 个简单序列重复（SSR）和序列特征扩增区（SCAR）标记对两个亲本进行了多态性调查。观察结果显示，近 50%的杂交种属于抗性类别，50%属于易感类别。标记辅助筛选的结果证实，38 个 F1 植株携带疮痂病抗性基因，其余 32 个 F1 植株则缺乏该基因。这 38 个基因型上抗疮痂病的杂交种被选作柱状植株和非柱状植株进行进一步鉴定。根据选择标准，21 个个体被归类为柱状植株，其余 17 个个体被归类为非柱状植株。表型筛选之后，使用 Co 基因分子标记对 F1 进行筛选。通过扩增 Co 特异性标记，在群体中发现了柱状特异性片段，并符合预期的 1:1 孟德尔比例。结果发现，18 个抗疮痂病的 F1 代杂交种携带 Co 基因，其余 20 个则不携带柱状生长习性基因。本研究确定的基因特异性引物可直接用于在短时间内筛选大量苹果种质，以培育抗苹果疮痂病的品种和具有柱状生长习性的品种。经证实具有疮痂病抗性和柱状生长习性的杂交种可迅速用作抗疮痂病的柱状栽培品种。
期刊介绍：
Plant Biotechnology Reports publishes original, peer-reviewed articles dealing with all aspects of fundamental and applied research in the field of plant biotechnology, which includes molecular biology, genetics, biochemistry, cell and tissue culture, production of secondary metabolites, metabolic engineering, genomics, proteomics, and metabolomics. Plant Biotechnology Reports emphasizes studies on plants indigenous to the Asia-Pacific region and studies related to commercialization of plant biotechnology. Plant Biotechnology Reports does not exclude studies on lower plants including algae and cyanobacteria if studies are carried out within the aspects described above.
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