Francisca B Stefany Aires do Nascimento, Lívia Gurgel do Amaral Valente Sá, João B de Andrade Neto, Lisandra Juvêncio da Silva, Daniel Sampaio Rodrigues, Vitória P de Farias Cabral, Amanda Dias Barbosa, Lara E Almeida Moreira, Camille R Braga Vasconcelos, Bruno Coêlho Cavalcanti, Maria E França Rios, Jacilene Silva, Emmanuel Silva Marinho, Helcio Silva Dos Santos, Jacó Rl de Mesquita, Marina Duarte Pinto Lobo, Manoel Odorico de Moraes, Hélio V Nobre Júnior, Cecília Rocha da Silva
{"title":"Antimicrobial activity of hydralazine against methicillin-resistant and methicillin-susceptible <i>Staphylococcus aureus</i>.","authors":"Francisca B Stefany Aires do Nascimento, Lívia Gurgel do Amaral Valente Sá, João B de Andrade Neto, Lisandra Juvêncio da Silva, Daniel Sampaio Rodrigues, Vitória P de Farias Cabral, Amanda Dias Barbosa, Lara E Almeida Moreira, Camille R Braga Vasconcelos, Bruno Coêlho Cavalcanti, Maria E França Rios, Jacilene Silva, Emmanuel Silva Marinho, Helcio Silva Dos Santos, Jacó Rl de Mesquita, Marina Duarte Pinto Lobo, Manoel Odorico de Moraes, Hélio V Nobre Júnior, Cecília Rocha da Silva","doi":"10.2217/fmb-2023-0160","DOIUrl":null,"url":null,"abstract":"<p><p><b>Background:</b> <i>Staphylococcus aureus</i> is a human pathogen responsible for high mortality rates. The development of new antimicrobials is urgent. <b>Materials & methods:</b> The authors evaluated the activity of hydralazine along with its synergism with other drugs and action on biofilms. With regard to action mechanisms, the authors evaluated cell viability, DNA damage and molecular docking. <b>Results:</b> MIC and minimum bactericidal concentration values ranged from 128 to 2048 μg/ml. There was synergism with oxacillin (50%) and vancomycin (25%). Hydralazine reduced the viability of biofilms by 50%. After exposure to hydralazine 2× MIC, 58.78% of the cells were unviable, 62.07% were TUNEL positive and 27.03% presented damage in the comet assay (p < 0.05). Hydralazine showed affinity for DNA gyrase and TyrRS. <b>Conclusion:</b> Hydralazine is a potential antibacterial.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.2217/fmb-2023-0160","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/31 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0
Abstract
Background:Staphylococcus aureus is a human pathogen responsible for high mortality rates. The development of new antimicrobials is urgent. Materials & methods: The authors evaluated the activity of hydralazine along with its synergism with other drugs and action on biofilms. With regard to action mechanisms, the authors evaluated cell viability, DNA damage and molecular docking. Results: MIC and minimum bactericidal concentration values ranged from 128 to 2048 μg/ml. There was synergism with oxacillin (50%) and vancomycin (25%). Hydralazine reduced the viability of biofilms by 50%. After exposure to hydralazine 2× MIC, 58.78% of the cells were unviable, 62.07% were TUNEL positive and 27.03% presented damage in the comet assay (p < 0.05). Hydralazine showed affinity for DNA gyrase and TyrRS. Conclusion: Hydralazine is a potential antibacterial.
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