In vitro comparative evaluation of apical leakage using a bioceramic sealer with three different obturating techniques: A glucose leakage model

Tanvi S Agrawal, Shishir Singh, Rajesh S. Podar, Gaurav P. Kulkarni, Anuprita Gadkari, Navin S Agarwal
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Abstract

Bioceramic sealers have improved sealing ability by forming an interfacial apatite layer that chemically bonds the sealer and radicular dentin thus decrease apical leakage. This study aims to evaluate and compare the apical leakage of Cerafill RCS bioceramic sealer and gutta percha when used with three different obturating techniques. Thirty-four extracted single-rooted premolars were decoronated and prepared up to size F3. Then, the specimens were randomly divided into 3 experimental groups (n = 10) cold lateral obturation technique, warm vertical obturation technique, single-cone obturation technique, positive and negative control groups (n = 2), according to the obturation technique used along with a bioceramic sealer. To evaluate apical leakage, all specimens were mounted in a glucose leakage model and assessed at 7 and 14 days using an ultraviolet-visible spectrophotometer. The results were subjected to ANOVA/Kruskal–Wallis ANOVA; followed by post hoc analysis using Bonferroni correction. Significant differences were found in the cumulative leakage of all the three experimental groups. Significantly higher leakage was found in groups obturated using single-cone obturation technique as compared to warm vertical compaction technique at both 7 and 14 days. Warm vertical compaction showed a better sealing result than single-cone obturation techniques at all observation periods.
使用生物陶瓷封闭剂和三种不同闭合技术对根尖渗漏进行体外比较评估:葡萄糖渗漏模型
生物陶瓷封闭剂通过形成界面磷灰石层,将封闭剂和根尖牙本质化学粘结在一起,从而提高了封闭能力,减少了根尖渗漏。 本研究旨在评估和比较 Cerafill RCS 生物陶瓷封闭剂和古塔胶在使用三种不同的闭合技术时的根尖渗漏情况。 我们对 34 颗拔出的单根前臼齿进行了装饰,并制备成 F3 尺寸。然后,根据与生物陶瓷封闭剂一起使用的封闭技术,将标本随机分为 3 个实验组(n = 10):冷侧封闭技术组、温垂直封闭技术组、单锥封闭技术组、阳性对照组和阴性对照组(n = 2)。为了评估根尖渗漏,将所有标本安装在葡萄糖渗漏模型中,并在 7 天和 14 天时使用紫外可见分光光度计进行评估。 对结果进行方差分析/Kruskal-Wallis 方差分析,然后使用 Bonferroni 校正进行事后分析。 所有三个实验组的累积渗漏量均存在显著差异。在 7 天和 14 天内,使用单锥套扎技术套扎的组比使用温热垂直压实技术套扎的组渗漏率明显更高。 在所有观察期内,温热垂直压合技术的密封效果均优于单锥体阻塞技术。
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