Study of Antibacterial Drugs’ Nephrotoxicity in the RPTEC Cell Line

Antibiotics and Chemotherapy Pub Date : 2024-01-15 DOI:10.37489/0235-2990-2023-68-9-10-42-45

V. Evteev, I. S. Semenova, N. Bunyatyan, A. Prokofiev, V. G. Kukes

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Abstract

The created cell line of human proximal renal tubules RPTEC/TERT1 showed maximum compliance with primary human RPTEC, which makes it optimal for use in studies of the nephrotoxic properties of xenobiotics. Transepithelial resistance measurement (TEER) is a valuable non-invasive method that can be used to quantify the integrity of the cell barrier at various stages of cell growth and differentiation, as well as to predict the toxicity and permeability of drugs.The aim of the study was to examine the dynamics of changes in transepithelial resistance in the model of the RPTEC/TERT1 cell line during incubation with drugs with nephrotoxic effects.Material and methods. The human proximal renal tubule cell line RPTEC/TERT was obtained from the ATCC cell culture bank. Cells at passage 14 were used in the experiment. The following drugs were studied: cisplatin: 2.5 mcg/ml; vancomycin: 50 mcg/ml; doripenem — 20mcg/ml; cefepim — 150mcg/ml. 4–5 repetitions were performed for each concentration of the drug in the experiment. THEER measurements were carried out 4–5 times for each well.Results. Cisplatin, vancomycin, doripenem, and cefepim in the concentrations used do not show a cytotoxic effect on the RPTEC cell line according to TEER dynamics.

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在 RPTEC 细胞系中研究抗菌药物的肾毒性

创建的人类近端肾小管细胞系 RPTEC/TERT1 显示出与原代人类 RPTEC 最大的顺应性，这使其成为研究异种生物肾毒性特性的最佳选择。跨上皮阻力测量（TEER）是一种有价值的非侵入性方法，可用于量化细胞生长和分化不同阶段细胞屏障的完整性，以及预测药物的毒性和渗透性。本研究的目的是考察 RPTEC/TERT1 细胞系模型在与具有肾毒性作用的药物孵育期间跨上皮阻力的动态变化。人近端肾小管细胞系 RPTEC/TERT 从 ATCC 细胞库中获得。实验中使用的是 14 胞龄的细胞。研究药物如下：顺铂：2.5 mcg/ml；万古霉素：50 mcg/ml；多立培南 - 20mcg/ml；头孢匹灵 - 150mcg/ml。实验中每种浓度的药物重复进行 4-5 次。每孔进行 4-5 次 THEER 测量。根据 TEER 动态分析，所使用浓度的顺铂、万古霉素、多立培南和头孢匹姆对 RPTEC 细胞系没有细胞毒性作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Antibiotics and Chemotherapy

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