Morphological and SSR Marker based Selection for an Elite YMV Resistant Breeding Line from a Segregating Population of Soybean [Glycine max (L.) Merrill]

LEGUME RESEARCH - AN INTERNATIONAL JOURNAL Pub Date : 2024-01-24 DOI:10.18805/lr-5238

D. Sahu, B. S. Gill, A. Sirari, M. Pradhan, N. Sahu, A. Kumar, A. Rani

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Abstract

Background: JS335 is a leading variety of soybean in central India with high stability, but YMV susceptible. These leads to the incorporation of the YMV resistance in the elite genotypes of soybean variety JS335. Methods: 45 breeding lines obtained from a cross between two parents SL525 (YMV resistant) and JS335 (YMV susceptible) along with two parents and a check SL958 were analysed for diversity using Mahalanobis D2 and 19 polymorphic SSR markers. These lines were evaluated for yield and component traits and YMV, during 2020 at two locations viz. Ludhiana and Gurdaspur, India. Result: Diversity analysis based on Mahalanobis D2 statistics divided genotypes into five clusters. The maximum (288464.1) and minimum (15103.7) inter-cluster distance were observed between clusters 4 and 5 and clusters 2 and 5, respectively. SSR marker analysis grouped a total of 48 genotypes into three major clusters. The mean for agronomic traits of genotypes was categorised and SLJS 41-2 was found YMV resistant and exhibited a high yield (32.1 g/plant). The contribution of JS 335 towards SLJS 41-2 was found to be 62.5%. This line could further be tested at multilocation to evaluate adaptability and subjected to different yield trials for varietal release or used as a potential YMV donor.

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基于形态学和 SSR 标记从大豆 [Glycine max (L.) Merrill] 分离群体中选育抗 YMV 的精英品系

背景：JS335 是印度中部大豆的主要品种，具有高稳定性，但易感 YMV。因此，需要在 JS335 大豆品种的优良基因型中加入抗 YMV 的基因。方法：使用 Mahalanobis D2 和 19 个多态 SSR 标记分析了从两个亲本 SL525（抗 YMV）和 JS335（易感 YMV）以及两个亲本和一个对照 SL958 杂交获得的 45 个育种品系的多样性。2020 年期间，在印度卢迪亚纳和古达斯布尔两个地点对这些品系的产量、成分性状和 YMV 进行了评估。结果基于 Mahalanobis D2 统计的多样性分析将基因型划分为五个聚类。簇间距离最大（288464.1），最小（15103.7），分别位于簇 4 和簇 5 之间以及簇 2 和簇 5 之间。SSR 标记分析将 48 个基因型分为三大类。对基因型的农艺性状平均值进行了分类，发现 SLJS 41-2 抗 YMV，产量高（32.1 克/株）。JS 335 对 SLJS 41-2 的贡献率为 62.5%。该品系可进一步进行多地测试，以评估其适应性，并进行不同的产量试验，以发布品种或用作潜在的 YMV 供体。

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LEGUME RESEARCH - AN INTERNATIONAL JOURNAL

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