P53-induced GAP-43 Upregulation in Primary Cortical Neurons of Rats.

IF 1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Protein and Peptide Letters Pub Date : 2024-01-01 DOI:10.2174/0109298665263864231221071712

Tianxia Li, Yuexin Jia, Junxian Fu, Zhuo Fu, Zhidong Qiao, Xiaoyang Liu, Ting Lv, Rong Tang, Guanglu Yang

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引用次数: 0

Abstract

Objectives: In this study, we employed an in vitro culturing technique to investigate the impact of p53 on the modulation of growth-associated protein-43 (GAP-43) within the primary cortical neurons of rat specimens.

Methods: (1) Within the first 24 hours after birth, the bilateral cortex was extracted from newborn Wistar rats and primary cortical neurons were cultured and identified. (2) The changes in the mRNA and protein expressions of GAP-43 induced by p53 in rat primary cortical neurons cultured in vitro were identified utilizing real-time polymerase chain reaction and western blot techniques.

Results: (1) Lentiviral transfection of p53 within primary cortical neurons of rats elicited elevated levels of both mRNA and protein expressions of GAP-43, consequently culminating in a noteworthy augmentation of p53 expression. (2) The introduction of a p53 inhibitor in rat primary cortical neurons resulted in a reduction in both mRNA and protein expressions of GAP-43.

Conclusion: Within primary rat cortical neurons, p53 has the potential to prompt an augmentation in both the transcriptional and protein expression levels of the GAP-43 protein.

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大鼠初级皮层神经元中 P53 诱导的 GAP-43 上调

研究目的方法：1）在新生Wistar大鼠出生后24小时内，提取其双侧皮层，培养并鉴定大鼠原代皮层神经元。2）利用实时聚合酶链式反应和 Western 印迹技术鉴定 p53 诱导的 GAP-43 mRNA 和蛋白表达在体外培养的大鼠原代皮层神经元中的变化：1）慢病毒转染大鼠原代皮质神经元中的 p53 可引起 GAP-43 mRNA 和蛋白表达水平的升高，从而导致 p53 表达的显著增强。2）在大鼠原代皮层神经元中引入 p53 抑制剂会导致 GAP-43 的 mRNA 和蛋白表达量减少：结论：在原代大鼠大脑皮层神经元中，p53 有可能促使 GAP-43 蛋白的转录和蛋白表达水平增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Protein and Peptide Letters 生物-生化与分子生物学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Protein & Peptide Letters publishes letters, original research papers, mini-reviews and guest edited issues in all important aspects of protein and peptide research, including structural studies, advances in recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, and drug design. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallization and preliminary structure determination of biologically important proteins are considered only if they include significant new approaches or deal with proteins of immediate importance, and preliminary structure determinations of biologically important proteins. Purely theoretical/review papers should provide new insight into the principles of protein/peptide structure and function. Manuscripts describing computational work should include some experimental data to provide confirmation of the results of calculations. Protein & Peptide Letters focuses on: Structure Studies Advances in Recombinant Expression Drug Design Chemical Synthesis Function Pharmacology Enzymology Conformational Analysis Immunology Biotechnology Protein Engineering Protein Folding Sequencing Molecular Recognition Purification and Analysis