Dynamic immobilization of bacterial cells on biofilm in a polyester nonwoven chemostat

IF 4.3 3区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioresources and Bioprocessing Pub Date : 2024-01-24 DOI:10.1186/s40643-024-00732-0

Chao-Lei Zhang, Chao Wang, Yue-Sheng Dong, Ya-Qin Sun, Zhi-Long Xiu

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Abstract

Cell immobilization plays an important role in biocatalysis for high-value products. It is necessary to maintain the viability of immobilized cells for bioconversion using viable cells as biocatalysts. In this study, a novel polyester nonwoven chemostat was designed for cell immobilization to investigate biofilm formation and the dynamic balance between adsorption and desorption of cells on polyester nonwoven. The polyester nonwoven was suitable for cell immobilization, and the cell numbers on the polyester nonwoven can reach 6.5 ± 0.38 log CFU/mL. After adding the polyester nonwoven to the chemostat, the fluctuation phenomenon of free bacterial cells occurred. The reason for this phenomenon was the balance between adsorption and desorption of bacterial cells on the polyester nonwoven. Bacterial cells could adhere to the surface of polyester nonwoven via secreting extracellular polymeric substances (EPS) to form biofilms. As the maturation of biofilms, some dead cells inside the biofilms can cause the detachment of biofilms. This process of continuous adsorption and desorption of cells can ensure that the polyester nonwoven chemostat has lasting biological activity.

Graphical Abstract

Abstract Image

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将细菌细胞动态固定在聚酯无纺布恒温器的生物膜上

细胞固定化在生产高价值产品的生物催化过程中发挥着重要作用。使用有活力的细胞作为生物催化剂进行生物转化时，必须保持固定化细胞的活力。本研究设计了一种用于细胞固定的新型聚酯无纺布恒温器，以研究生物膜的形成以及细胞在聚酯无纺布上吸附和解吸之间的动态平衡。聚酯无纺布适用于细胞固定，聚酯无纺布上的细胞数量可达 6.5 ± 0.38 log CFU/mL。在恒温器中加入聚酯无纺布后，出现了游离细菌细胞的波动现象。造成这种现象的原因是细菌细胞在聚酯无纺布上的吸附和解吸之间的平衡。细菌细胞可通过分泌胞外聚合物物质（EPS）附着在聚酯无纺布表面，形成生物膜。随着生物膜的成熟，生物膜内的一些死亡细胞会导致生物膜脱落。这种细胞不断吸附和脱附的过程可确保聚酯无纺布恒温器具有持久的生物活性。

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来源期刊

Bioresources and Bioprocessing BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

7.20

自引率

8.70%

发文量

118

审稿时长

13 weeks

期刊介绍： Bioresources and Bioprocessing (BIOB) is a peer-reviewed open access journal published under the brand SpringerOpen. BIOB aims at providing an international academic platform for exchanging views on and promoting research to support bioresource development, processing and utilization in a sustainable manner. As an application-oriented research journal, BIOB covers not only the application and management of bioresource technology but also the design and development of bioprocesses that will lead to new and sustainable production processes. BIOB publishes original and review articles on most topics relating to bioresource and bioprocess engineering, including: -Biochemical and microbiological engineering -Biocatalysis and biotransformation -Biosynthesis and metabolic engineering -Bioprocess and biosystems engineering -Bioenergy and biorefinery -Cell culture and biomedical engineering -Food, agricultural and marine biotechnology -Bioseparation and biopurification engineering -Bioremediation and environmental biotechnology