Correlation of single nucleotide polymorphisms in the AGT gene with susceptibility to systemic lupus erythematosus in Northeast China

IF 2.3 4区医学 Q3 GENETICS & HEREDITY

International Journal of Immunogenetics Pub Date : 2024-01-24 DOI:10.1111/iji.12655

Huitao Wu, Xuan Zhang, Guiling Lin, Qi Zhang, Ziman He, Zhe Wang, Wenlu Xu, Xiyu Yin, Linglan Su, Yanping Zhuang, Aimin Gong

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Abstract

To investigate the correlation between susceptibility to systemic lupus erythematosus (SLE) and single nucleotide polymorphisms (SNPs) rs699, rs4762 and rs1926723 in the AGT gene in the population of Northeast China, while also introducing a new method for early detection of SLE. A total of 856 cases of SLE patients and healthy volunteers who attended the First Affiliated Hospital of Harbin Medical University from January 2020 to December 2022 were recruited. Clinical information and biood samples were collected from particpants in this study. SNaPshot sequencing technology was used to sequence the bases of the rs699, rs4762 and rs1926723 in the AGT gene. The genetic stability of SNPs was analysed by means of Hardy–Weinberg (HWE) genetic equilibrium. The study examined the correlation between genetically stable SNPs and susceptibility to SLE using logistic regression analysis.

Rs699 did not adhere to the principles of the HWE genetic equilibrium (p < .01). Conversely, both rs4762 and rs1926723 conformed to the HWE genetic equilibrium (p > .05). However, no significant differences in genotypes and alleles frequencies of the rs4762 were observed between the two groups (p > .05). Furthermore, there was a significant difference in the distribution of AG, GG genotypes frequency and G allele frequency at the rs1926723 between the two groups (p < .001). Individuals with AG and GG genotypes and the G allele had a significantly lower frequency of SLE, indicating a potential genetic protective factor against susceptibility to the SLE.

The SNPs rs1926723 may be linked to the susceptibility to SLE, and the AG, GG genotypes and the G allele may be important protective factors for the development of SLE in Northeast China.

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中国东北地区 AGT 基因单核苷酸多态性与系统性红斑狼疮易感性的相关性。

研究东北地区人群系统性红斑狼疮（SLE）易感性与 AGT 基因中单核苷酸多态性（SNPs）rs699、rs4762 和 rs1926723 之间的相关性，同时介绍一种早期检测系统性红斑狼疮的新方法。本次研究共招募了 856 例系统性红斑狼疮患者和健康志愿者，他们均于 2020 年 1 月至 2022 年 12 月在哈尔滨医科大学附属第一医院就诊。本研究收集了参与者的临床信息和生物样本。采用 SNaPshot 测序技术对 AGT 基因中的 rs699、rs4762 和 rs1926723 进行碱基测序。通过哈代-温伯格（HWE）遗传平衡分析了 SNPs 的遗传稳定性。研究采用逻辑回归分析法检验了遗传稳定的 SNP 与系统性红斑狼疮易感性之间的相关性。Rs699不符合HWE遗传平衡原则（p .05）。然而，两组之间的 rs4762 基因型和等位基因频率没有明显差异（p > .05）。此外，rs1926723 的 AG、GG 基因型频率和 G 等位基因频率的分布在两组间也有显著差异（p.05）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Immunogenetics 医学-免疫学

CiteScore

4.70

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The International Journal of Immunogenetics (formerly European Journal of Immunogenetics) publishes original contributions on the genetic control of components of the immune system and their interactions in both humans and experimental animals. The term ''genetic'' is taken in its broadest sense to include studies at the evolutionary, molecular, chromosomal functional and population levels in both health and disease. Examples are: -studies of blood groups and other surface antigens- cell interactions and immune response- receptors, antibodies, complement components and cytokines- polymorphism- evolution of the organisation, control and function of immune system components- anthropology and disease associations- the genetics of immune-related disease: allergy, autoimmunity, immunodeficiency and other immune pathologies- All papers are seen by at least two independent referees and only papers of the highest quality are accepted.