Effects of flusulfamide on spore germination of Plasmodiophora brassicae

IF 1.5 4区 农林科学 Q2 ENTOMOLOGY
Taito Sasaki, Ryohei Naito, Toshiaki Ohara, Kosei Sakane, Shuhei Tanaka, Kazunori Sasaki, Aoi Osato, Yuki Nomura, Shin-ichi Ito
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引用次数: 0

Abstract

Flusulfamide inhibits germination of Plasmodiophora brassicae resting spores to suppress clubroot disease, but its mechanism of action on the germination of P. brassicae resting spores remains unclear. In this study, P. brassicae resting spores were treated with flusulfamide and visualized using transmission electron microscopy (TEM). The gene expression of P. brassicae resting spores was analyzed using RT-PCR, followed by immunoblotting analysis. TEM results revealed that flusulfamide suppressed the primary zoosporogenesis of P. brassicae resting spores during the early phase, and RT-PCR results revealed that flusulfamide affected the gene expression during the germination of the resting spores. Immunoblot and RT-qPCR analyses revealed that PbCyp3, an immunophilin (peptidyl-prolyl-isomerase) gene, was highly expressed, resulting in the unusual accumulation of PbCYP3 protein in P. brassicae resting spores immediately after treatment with flusulfamide. This suggests that flusulfamide may cause aberrant folding of proteins involved in primary zoosporogenesis, thereby inhibiting germination.

Abstract Image Fullsize Image
氟磺酰胺对黄铜质菌孢子萌发的影响
氟磺酰胺可抑制黄刺浆孢属(Plasmodiophora brassicae)静止孢子的萌发,从而抑制球根病,但其对黄刺浆孢属静止孢子萌发的作用机制仍不清楚。在这项研究中,用氟磺酰胺处理了黄铜病菌的静止孢子,并用透射电子显微镜(TEM)进行了观察。使用 RT-PCR 分析了黄铜苣苔静止孢子的基因表达,然后进行了免疫印迹分析。透射电子显微镜结果显示,氟磺胺抑制了铜绿微囊藻静止孢子早期的初级动物孢子发生;RT-PCR结果显示,氟磺胺影响了静止孢子萌发过程中的基因表达。免疫印迹和 RT-qPCR 分析表明,PbCyp3(一种免疫嗜蛋白(肽基-脯氨酰-异构酶)基因)表达量很高,这导致在使用氟磺酰胺处理后,黄刺苣苔静止孢子中的 PbCYP3 蛋白会立即异常积累。这表明氟磺酰胺可能会导致参与初级动物孢子发生的蛋白质折叠异常,从而抑制发芽。
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来源期刊
Journal of Pesticide Science
Journal of Pesticide Science 农林科学-昆虫学
CiteScore
4.30
自引率
4.20%
发文量
28
审稿时长
18-36 weeks
期刊介绍: The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.
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