Association of CC-chemokine ligand-2 gene polymorphisms with leprosy reactions

IF 2.6 4区医学 Q3 IMMUNOLOGY

Microbes and Infection Pub Date : 2024-05-01 DOI:10.1016/j.micinf.2024.105298

Sanjay Kumar Biswas , Keshar Kunja Mohanty , Vandana Singh , Mohan Natrajan , Mamta Arora , Joy Kumar Chakma , Srikanth Prasad Tripathy

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引用次数: 0

Abstract

Background

C–C motif chemokine ligand 2, a gene that codes for a protein involved in inflammation. Certain SNPs in the CCL2 gene have been studied for their potential associations with susceptibility to various diseases. These SNPs may affect the production and function of the CCL2 protein, which is involved in the recruitment of immune cells to the site of inflammation. Variations in CCL2 may influence the immune response to Mycobacterium leprae infection.

Objective

To investigate the association of the C–C motif chemokine ligand-2 single nucleotide polymorphisms with leprosy.

Methods

CCL2 single nucleotide polymorphisms were analyzed in a total of 975 leprosy patients and 357 healthy controls. Of those, 577 leprosy and 288 healthy controls were analyzed by PCR-RFLP for CCL2 -2518 A>G, 535 leprosy and 290 controls for CCL2 -362 G>C, 295 leprosy and 240 controls for CCL2 -2134 T>G, 325 leprosy and 288 controls for CCL2 -1549 A>T SNPs by melting curve analysis using hybridization probe chemistry and detection by fluorescence resonance energy transfer (FRET) technique in Realtime PCR. The levels of CCL2, IL-12p70, IFN-γ, TNF-α, and TGF-β were estimated in sera samples and correlated with CCL2 genotypes.

Results

The frequency of the GCT (-2518 A>G, -362 G>C, -2134 T>G) haplotype is observed to be higher in leprosy patients compared to healthy controls (P = 0.04). There was no significant difference observed in genotypic frequencies between leprosy patients and healthy controls {(-2518 A>G, p = 0.53), (-362 G>C, p = 0.01), (-2134 T>G, p = 0.10)}. G allele at the -2134 site is predominant in leprosy (borderline) without any reaction (8 %) compared to borderline patients with RR reactions (2.1 %) (P = 0.03). GG genotype (p = 0.008) and G allele at -2518 (p = 0.030) of the CCL 2 gene were found to be associated with patients with ENL reaction. An elevated level of serum CCL2 was observed in leprosy patients with the -2518 AA and AG genotypes (p = 0.0001).

Conclusions

G allele and GG genotype at the CCL2 -2518 site are associated with a risk of ENL reactions.

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CC-Chemokine Ligand-2 基因多态性与麻风病反应的关系

背景 C-C motif趋化因子配体 2 是一种编码参与炎症的蛋白质的基因。CCL2 基因中的某些 SNPs 可能与各种疾病的易感性有关。这些 SNPs 可能会影响 CCL2 蛋白的产生和功能，而 CCL2 蛋白参与将免疫细胞招募到炎症部位。方法对975名麻风病人和357名健康对照者的CCL2单核苷酸多态性进行分析。其中，577 名麻风病人和 288 名健康对照者通过 PCR-RFLP 分析了 -2518 A>G，535 名麻风病人和 290 名对照者通过 PCR-RFLP 分析了 CCL2 -362 G>C，295 名麻风病人和 240 名对照者通过 PCR-RFLP 分析了 -2134 T>G，325 名麻风病人和 288 名对照者通过 PCR-RFLP 分析了 1549 A>T SNPs。结果与健康对照组相比，麻风病人的 GCT（-2518 A>G、-362 G>C、-2134 T>G）单倍型频率较高（P=0.04）。麻风病人与健康对照组的基因型频率无明显差异{（-2518 A>G，P=0.53），（-362 G>C，P=0.01），（-2134 T>G，P= 0.10）}。与有 RR 反应的边缘型病人（2.1%）相比，无任何反应的边缘型麻风病人（8%）中，-2134 位点的 G 等位基因占优势（P=0.03）。研究发现，CCL 2 基因的 GG 基因型（P=0.008）和 -2518 位点的 G 等位基因（P=0.030）与 ENL 反应患者有关。结论 CCL2 -2518位点的G等位基因和GG基因型与ENL反应风险有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbes and Infection 医学-病毒学

CiteScore

12.60

自引率

1.70%

发文量

审稿时长

40 days

期刊介绍： Microbes and Infection publishes 10 peer-reviewed issues per year in all fields of infection and immunity, covering the different levels of host-microbe interactions, and in particular: the molecular biology and cell biology of the crosstalk between hosts (human and model organisms) and microbes (viruses, bacteria, parasites and fungi), including molecular virulence and evasion mechanisms. the immune response to infection, including pathogenesis and host susceptibility. emerging human infectious diseases. systems immunology. molecular epidemiology/genetics of host pathogen interactions. microbiota and host "interactions". vaccine development, including novel strategies and adjuvants. Clinical studies, accounts of clinical trials and biomarker studies in infectious diseases are within the scope of the journal. Microbes and Infection publishes articles on human pathogens or pathogens of model systems. However, articles on other microbes can be published if they contribute to our understanding of basic mechanisms of host-pathogen interactions. Purely descriptive and preliminary studies are discouraged.