Rapid antibiotic susceptibility testing (RAST) of blood cultures in enterobacteria with inducible chromosomal AmpC-type β-lactamase

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES

Enfermedades infecciosas y microbiologia clinica Pub Date : 2024-04-01 DOI:10.1016/j.eimc.2023.12.008

Blanca Carrasco, Gloria Zaragoza, David M. Arana, Yolanda Hernández-Hermida, Juan-Ignacio Alós

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Abstract

Introduction

Early and adequate treatment of bloodstream infections decreases patient morbidity and mortality. The objective is to develop a preliminary method for rapid antibiotic susceptibility testing (RAST) in enterobacteria with inducible chromosomal AmpC.

Methods

RAST was performed directly on spiked blood cultures of 49 enterobacteria with inducible chromosomal AmpC. Results were read at 4, 6 and 8 h of incubation. Commercial broth microdilution was considered the reference method. Disks of 10 antibiotics were evaluated.

Results

The proportion of readable tests at 4 h was 85%. All RAST could be read at 6 and 8 h.

For most antibiotics, the S or R result at 4, 6 and 8 h was greater than 80% after tentative breakpoints were established and Area of Technical Uncertainty was defined.

Conclusions

This preliminary method seems to be of practical use, although it should be extended to adjust the breakpoints and differentiate them by species.

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对带有诱导性染色体 AmpC 型 β-内酰胺酶的肠杆菌血液培养物进行快速抗生素敏感性测试 (RAST)

引言及早、充分治疗血流感染可降低患者的发病率和死亡率。方法直接对 49 种具有诱导性染色体 AmpC 的肠道细菌的血液培养物进行快速抗生素敏感性检测（RAST）。结果在培养 4、6 和 8 小时后读取。商用肉汤微量稀释法被视为参考方法。结果 4 小时可读取结果的比例为 85%。对于大多数抗生素来说，在确定了暂定断点和技术不确定区域后，4、6 和 8 小时的 S 或 R 结果均大于 80%。结论这种初步方法似乎很实用，但应加以扩展，以调整断点并按物种加以区分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Enfermedades infecciosas y microbiologia clinica 医学-传染病学

CiteScore

2.10

自引率

8.00%

发文量

194

审稿时长

29 days

期刊介绍： Hoy está universalmente reconocida la renovada y creciente importancia de la patología infecciosa: aparición de nuevos agentes patógenos, de cepas resistentes, de procesos con expresión clínica hasta ahora desconocida, de cuadros de una gran complejidad. Paralelamente, la Microbiología y la Infectología Clínicas han experimentado un gran desarrollo como respuesta al reto planteado por la actual patología infecciosa. Enfermedades Infecciosas y Microbiología Clínica es la Publicación Oficial de la Sociedad Española SEIMC. Cumple con la garantía científica de esta Sociedad, la doble función de difundir trabajos de investigación, tanto clínicos como microbiológicos, referidos a la patología infecciosa, y contribuye a la formación continuada de los interesados en aquella patología mediante artículos orientados a ese fin y elaborados por autores de la mayor calificación invitados por la revista.