Optimized extraction of phycobiliproteins from Arthrospira platensis: quantitative and qualitative assessment of C-Phycocyanin, Allophycocyanin, and Phycoerythrin
{"title":"Optimized extraction of phycobiliproteins from Arthrospira platensis: quantitative and qualitative assessment of C-Phycocyanin, Allophycocyanin, and Phycoerythrin","authors":"Ibtissam lijassi, Fadia Arahou, Sabre Taher Harane Koudi, Anass Wahby, Souad Benaich, Laila Rhazi, Imane Wahby","doi":"10.1007/s43153-023-00428-6","DOIUrl":null,"url":null,"abstract":"<p>Phycobiliproteins (PBPs) are light collecting pigments of cyanobacteria that attract growing interest for several industrial applications. Each step of the extraction process is crucial for yield, concentration and quality of obtained pigments. In the current work, we present an optimization scheme of major limiting steps for PBPs extraction from <i>Arthrospira platensis</i> biomass. As first step, the effects of pretreatment, extraction time, and separation conditions on the recovery of PBPs were compared. Subsequently, the influence of pH and concentration of the extraction buffer as well as the addition of preservatives (Polyethylene glycol (PEG), Magnesium chloride (MgCl<sub>2</sub>), and Calcium chloride (CaCl<sub>2</sub>)) was studied. In addition, the effect of the biomass type (dried vs wet) and its concentration in the extraction buffer was also investigated. Optimal extraction required the use of dry biomass at relatively low ratio (1:50, solvent:biomass), without previous treatment. The use of concentrated phosphate buffer (100 mM) at a neutral pH gave the highest PBPs recovery and concentration after 6 h of extraction followed with a separation at 6000 rpm during 15 min. Calcium chloride used at 1.5% improved by 30% both PBPs recovery and concentration in the crude extract. The optimized protocol allowed the recovery of 464.5 mg/g PBPs from <i>spirulina</i> biomass with concentration of 15.9 mg/ml. The crude PBPs obtained with this extraction method reduced the stable radical DPPH with a percentage scavenging activity of 86.45 ± 1.2%. This protocol could reduce both PBPs time and cost extraction and is easily scalable for industrial application.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s43153-023-00428-6","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Phycobiliproteins (PBPs) are light collecting pigments of cyanobacteria that attract growing interest for several industrial applications. Each step of the extraction process is crucial for yield, concentration and quality of obtained pigments. In the current work, we present an optimization scheme of major limiting steps for PBPs extraction from Arthrospira platensis biomass. As first step, the effects of pretreatment, extraction time, and separation conditions on the recovery of PBPs were compared. Subsequently, the influence of pH and concentration of the extraction buffer as well as the addition of preservatives (Polyethylene glycol (PEG), Magnesium chloride (MgCl2), and Calcium chloride (CaCl2)) was studied. In addition, the effect of the biomass type (dried vs wet) and its concentration in the extraction buffer was also investigated. Optimal extraction required the use of dry biomass at relatively low ratio (1:50, solvent:biomass), without previous treatment. The use of concentrated phosphate buffer (100 mM) at a neutral pH gave the highest PBPs recovery and concentration after 6 h of extraction followed with a separation at 6000 rpm during 15 min. Calcium chloride used at 1.5% improved by 30% both PBPs recovery and concentration in the crude extract. The optimized protocol allowed the recovery of 464.5 mg/g PBPs from spirulina biomass with concentration of 15.9 mg/ml. The crude PBPs obtained with this extraction method reduced the stable radical DPPH with a percentage scavenging activity of 86.45 ± 1.2%. This protocol could reduce both PBPs time and cost extraction and is easily scalable for industrial application.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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