Clinical significance of long non-coding RNA NORAD in rheumatoid arthritis.

IF 2 4区医学 Q3 RHEUMATOLOGY

Advances in Rheumatology Pub Date : 2024-01-18 DOI:10.1186/s42358-024-00349-z

Xueru Zhao, Weiyi Lin, Wenhui Zhou

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引用次数: 0

Abstract

Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease that may cause joint deformities and seriously affect the normal life of the patients. In order to enable patients to receive timely attention and treatment, this study developed new diagnostic markers by exploring the expression and molecular mechanism of the long non-coding RNA NORAD (NORAD) in RA.

Methods: Participants including 77 RA patients and 52 healthy persons were enrolled, and the corresponding clinical data and serum samples were obtained. The NORAD and miR-204-5p expression were detected by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). The content of inflammatory cytokines (IL-6, TNF-α) were determined through enzyme-linked immunosorbent assay (ELISA). Luciferase activity reporter assay demonstrated the association between NORAD and miR-204-5p. In addition, receiver operating characteristic (ROC) curve was used to evaluate the diagnostic efficacy of NORAD, and Pearson's correlation analysis was applied for the correlation analysis.

Results: NORAD was enriched in RA serum with high diagnostic value. Simultaneously, IL-6 and TNF-α levels were also upregulated (P < 0.001). The C-reactive protein (CRP), rheumatoid factor (RF), erythrocyte sedimentation rate (ESR) and anti-cyclic citrullinated peptide antibody (Anti-CCP) levels in RA patients were generally elevated (P < 0.001). NORAD was positively correlated with the levels of clinical indicators and inflammatory factors (P < 0.0001). Mechanistically, NORAD may affect the progression of RA by targeting and negatively regulating miR-204-5p.

Conclusions: There is a correlation between NORAD and the processes of RA, and NORAD has the potential to predict and diagnose the occurrence of RA.

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长非编码 RNA NORAD 在类风湿关节炎中的临床意义。

背景：类风湿关节炎（RA）是一种慢性自身免疫性疾病，可导致关节畸形，严重影响患者的正常生活。为了使患者得到及时的关注和治疗，本研究通过探讨长非编码 RNA NORAD（NORAD）在 RA 中的表达和分子机制，开发了新的诊断标记物：方法：研究人员招募了 77 名 RA 患者和 52 名健康人，并获得了相应的临床数据和血清样本。采用实时荧光定量聚合酶链反应（RT-qPCR）检测 NORAD 和 miR-204-5p 的表达。炎症细胞因子（IL-6、TNF-α）的含量通过酶联免疫吸附试验（ELISA）测定。荧光素酶活性报告实验证明了 NORAD 与 miR-204-5p 之间的关联。此外，还利用接收者操作特征曲线（ROC）评估了NORAD的诊断效果，并应用皮尔逊相关分析进行了相关性分析：结果：NORAD在RA血清中富集，具有很高的诊断价值。结果：NORAD在RA血清中富集，具有很高的诊断价值，同时IL-6和TNF-α水平也上调（PNORAD与RA的发病过程存在相关性，NORAD具有预测和诊断RA的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advances in Rheumatology Medicine-Rheumatology

CiteScore

4.00

自引率

4.30%

发文量

审稿时长

53 weeks

期刊介绍： Formerly named Revista Brasileira de Reumatologia, the journal is celebrating its 60th year of publication. Advances in Rheumatology is an international, open access journal publishing pre-clinical, translational and clinical studies on all aspects of paediatric and adult rheumatic diseases, including degenerative, inflammatory and autoimmune conditions. The journal is the official publication of the Brazilian Society of Rheumatology and welcomes original research (including systematic reviews and meta-analyses), literature reviews, guidelines and letters arising from published material.