Ultrastructural and immunohistochemical insights on the anti-glioma effects of a dual-drug cocktail in an in vivo experimental model.

IF 1.9 4区医学 Q3 INFECTIOUS DISEASES

Journal of Chemotherapy Pub Date : 2024-11-01 Epub Date: 2024-01-19 DOI:10.1080/1120009X.2024.2302741

Indrani Biswas, Daisy Precilla S, Shreyas S Kuduvalli, Bhavani K, Sivachandran R, Anitha T S

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引用次数: 0

Abstract

Glioma coined as 'butterfly tumor' exhibits intense heterogeneity at the molecular and cellular levels. Although, Temozolomide exerted a long-ranging and prevailing therapeutic effect against glioma, albeit it has provided modest survival outcome. Fucoidan, (marine brown algal derivative) has demonstrated potent anti-tumor effects including glioma. Nevertheless, there is paucity of studies conducted on Fucoidan to enhance the anti-glioma efficacy of Temozolomide. The present study aimed to explore the plausible synergistic anti-glioma efficacy of Fucoidan in combination with Temozolomide in an in vivo experimental model. The dual-drug combination significantly inhibited tumor growth in in vivo and prolonged the survival rate when compared with the other treatment and tumor-control groups, via down-regulation of inflammatory cascade- IL-6/T LR4 and JAK/STAT3 as per the immunohistochemistry findings. Furthermore, the ultrastructural analysis indicated that the combinatorial treatment had restored the normal neuronal architecture of glioma-induced rats. Overall, the dual-drug cocktail might enhance the therapeutic outcome in glioma patients.

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从超微结构和免疫组织化学角度揭示双药鸡尾酒在体内实验模型中的抗胶质瘤作用。

被称为 "蝴蝶瘤 "的胶质瘤在分子和细胞水平上表现出强烈的异质性。尽管替莫唑胺（Temozolomide）对脑胶质瘤具有长期和普遍的治疗效果，但其生存率并不高。褐藻糖胶（海洋褐藻衍生物）已显示出强大的抗肿瘤作用，包括胶质瘤。然而，有关褐藻糖胶增强替莫唑胺抗胶质瘤疗效的研究却很少。本研究旨在探讨褐藻糖胶与替莫唑胺在体内实验模型中的协同抗胶质瘤功效。免疫组化结果显示，与其他治疗组和肿瘤对照组相比，双药联用通过下调炎症级联--IL-6/T LR4和JAK/STAT3，明显抑制了体内肿瘤的生长，并延长了生存率。此外，超微结构分析表明，联合疗法恢复了胶质瘤诱导大鼠的正常神经元结构。总之，双药鸡尾酒疗法可提高胶质瘤患者的治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Chemotherapy 医学-药学

CiteScore

3.70

自引率

0.00%

发文量

144

审稿时长

6-12 weeks

期刊介绍： The Journal of Chemotherapy is an international multidisciplinary journal committed to the rapid publication of high quality, peer-reviewed, original research on all aspects of antimicrobial and antitumor chemotherapy. The Journal publishes original experimental and clinical research articles, state-of-the-art reviews, brief communications and letters on all aspects of chemotherapy, providing coverage of the pathogenesis, diagnosis, treatment, and control of infection, as well as the use of anticancer and immunomodulating drugs. Specific areas of focus include, but are not limited to: · Antibacterial, antiviral, antifungal, antiparasitic, and antiprotozoal agents; · Anticancer classical and targeted chemotherapeutic agents, biological agents, hormonal drugs, immunomodulatory drugs, cell therapy and gene therapy; · Pharmacokinetic and pharmacodynamic properties of antimicrobial and anticancer agents; · The efficacy, safety and toxicology profiles of antimicrobial and anticancer drugs; · Drug interactions in single or combined applications; · Drug resistance to antimicrobial and anticancer drugs; · Research and development of novel antimicrobial and anticancer drugs, including preclinical, translational and clinical research; · Biomarkers of sensitivity and/or resistance for antimicrobial and anticancer drugs; · Pharmacogenetics and pharmacogenomics; · Precision medicine in infectious disease therapy and in cancer therapy; · Pharmacoeconomics of antimicrobial and anticancer therapies and the implications to patients, health services, and the pharmaceutical industry.