Advances in high-throughput screening approaches for biosurfactants: current trends, bottlenecks and perspectives.

IF 8.1 2区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Critical Reviews in Biotechnology Pub Date : 2024-11-01 Epub Date: 2024-01-17 DOI:10.1080/07388551.2023.2290981
Muneer Ahmed Qazi, Irfan Ali Phulpoto, Qinhong Wang, Zongjie Dai
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引用次数: 0

Abstract

The market size of biosurfactants (BSs) has been expanding at an extremely fast pace due to their broad application scope. Therefore, the re-construction of cell factories with modified genomic and metabolic profiles for desired industrial performance has been an intriguing aspect. Typical mutagenesis approaches generate huge mutant libraries, whereas a battery of specific, robust, and cost-effective high-throughput screening (HTS) methods is requisite to screen target strains for desired phenotypes. So far, only a few specialized HTS assays have been developed for BSs that were successfully applied to obtain anticipated mutants. The most important milestones to reach, however, continue to be: specificity, sensitivity, throughput, and the potential for automation. Here, we discuss important colorimetric and fluorometric HTS approaches for possible intervention on automated HTS platforms. Moreover, we explain current bottlenecks in developing specialized HTS platforms for screening high-yielding producers and discuss possible perspectives for addressing such challenges.

生物表面活性剂高通量筛选方法的进展:当前趋势、瓶颈和前景。
由于生物表面活性剂(BS)的应用范围广泛,其市场规模一直在以极快的速度扩大。因此,如何利用经过改造的基因组和代谢特征重建细胞工厂,以实现所需的工业性能,一直是一个引人关注的问题。典型的诱变方法会产生庞大的突变体库,而要想筛选出目标菌株所需的表型,则需要一系列特异、稳健且经济高效的高通量筛选(HTS)方法。迄今为止,只有少数针对 BS 的专门 HTS 检测方法被成功应用于获得预期突变体。然而,最重要的里程碑仍然是:特异性、灵敏度、通量和自动化潜力。在此,我们将讨论重要的比色法和荧光法 HTS 方法,以便对自动化 HTS 平台进行可能的干预。此外,我们还解释了目前在开发用于筛选高产生产者的专用 HTS 平台方面存在的瓶颈,并讨论了应对这些挑战的可能前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Critical Reviews in Biotechnology
Critical Reviews in Biotechnology 工程技术-生物工程与应用微生物
CiteScore
20.80
自引率
1.10%
发文量
71
审稿时长
4.8 months
期刊介绍: Biotechnological techniques, from fermentation to genetic manipulation, have become increasingly relevant to the food and beverage, fuel production, chemical and pharmaceutical, and waste management industries. Consequently, academic as well as industrial institutions need to keep abreast of the concepts, data, and methodologies evolved by continuing research. This journal provides a forum of critical evaluation of recent and current publications and, periodically, for state-of-the-art reports from various geographic areas around the world. Contributing authors are recognized experts in their fields, and each article is reviewed by an objective expert to ensure accuracy and objectivity of the presentation.
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