Cyclin A2 Expression as Predictive Biomarker in Muscle-Invasive Upper Tract Urothelial Carcinoma.

IF 1.5 4区医学 Q3 UROLOGY & NEPHROLOGY

Urologia Internationalis Pub Date : 2024-01-01 Epub Date: 2024-01-15 DOI:10.1159/000536184

Margarete Teresa Walach, Katja Nitschke, Matthias Groß-Weege, Johannes Großhans, Lukas Wildner, Luca Pause, Jonas Jarczyk, Frederik Wessels, Manuel Neuberger, Karl-Friedrich Kowalewski, Maximilian Christian Kriegmair, Zoran V Popovic, Timo Gaiser, Thomas Stefan Worst, Philipp Nuhn

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引用次数: 0

Abstract

Introduction: The aim was to evaluate the prognostic value of altered Cyclin A2 (CCNA2) gene expression in upper tract urothelial carcinoma (UTUC) and to assess its predictive potential as a prognostic factor for overall survival (OS) and disease-free survival.

Methods: 62 patients who underwent surgical treatment for UTUC were included. Gene expression of CCNA2, MKI67, and p53 was analyzed by quantitative reverse transcriptase polymerase chain reaction. Survival analyses were performed using the Kaplan-Meier method and the log-rank test. For Cox regression analyses, uni- and multivariable hazard ratios were calculated. Spearman correlation was used to analyze correlation of CCNA2 expression with MKI67 and p53.

Results: The median age of the cohort was 73 years, and it consisted of 48 males (77.4%) and 14 females (22.6%). Patients with high CCNA2 expression levels showed longer OS (HR 0.33; 95% CI: 0.15-0.74; p = 0.0073). Multivariable Cox regression analyses identified CCNA2 overexpression (HR 0.37; 95% CI: 0.16-0.85; p = 0.0189) and grading G2 (vs. G3) (HR 0.39; 95% CI: 0.17-0.87; p = 0.0168) to be independent predictors for longer OS. CCNA2 expression correlated positively with MKI67 expression (Rho = 0.4376, p = 0.0005).

Conclusion: Low CCNA2 expression is significantly associated with worse OS. Thus, CCNA2 might serve as a potential biomarker in muscle-invasive UTUC and may be used to characterize a subset of patients having an unfavorable outcome and for future risk assessment scores.

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细胞周期蛋白 A2 表达作为肌肉浸润性上尿路上皮癌的预测性生物标记物。

引言方法：纳入62例接受手术治疗的UTUC患者。通过逆转录酶聚合酶链反应定量分析CCNA2、MKI67和p53的基因表达。采用卡普兰-梅耶法和对数秩检验进行生存分析。在 Cox 回归分析中，计算了单变量和多变量危险比。Spearman相关性用于分析CCNA2表达与MKI67和p53的相关性：组群的中位年龄为 73 岁，其中男性 48 人（77.4%），女性 14 人（22.6%）。CCNA2表达水平高的患者OS时间更长（HR 0.33; 95% CI 0.15 - 0.74; p = 0.0073）。多变量Cox回归分析发现，CCNA2过表达（HR 0.37；95% CI 0.16 - 0.85；p = 0.0189）和分级G2（vs. G3）（HR 0.39；95% CI 0.17 - 0.87；p = 0.0168）是延长OS的独立预测因素。CCNA2的表达与MKI67的表达呈正相关（Rho = 0.4376，p = 0.0005）：结论：CCNA2的低表达与较差的OS明显相关。因此，CCNA2可作为肌层浸润性UTUC的潜在生物标志物，并可用于鉴定预后不良的患者亚群，以及未来的风险评估评分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Urologia Internationalis 医学-泌尿学与肾脏学

CiteScore

3.30

自引率

6.20%

发文量

审稿时长

3-8 weeks

期刊介绍： Concise but fully substantiated international reports of clinically oriented research into science and current management of urogenital disorders form the nucleus of original as well as basic research papers. These are supplemented by up-to-date reviews by international experts on the state-of-the-art of key topics of clinical urological practice. Essential topics receiving regular coverage include the introduction of new techniques and instrumentation as well as the evaluation of new functional tests and diagnostic methods. Special attention is given to advances in surgical techniques and clinical oncology. The regular publication of selected case reports represents the great variation in urological disease and illustrates treatment solutions in singular cases.