Interaction mechanism between luteoloside and corn silk glycans and the synergistic role in hypoglycemic activity

IF 4.8 3区化学 Q1 CHEMISTRY, MEDICINAL

Natural Products and Bioprospecting Pub Date : 2024-01-16 DOI:10.1007/s13659-024-00428-0

Shihui Qin, Yanlang Li, Huiyan Shao, Yang Yu, Yina Yang, Yi Zeng, Jia Huang, Jiang-miao Hu, Liu Yang

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Abstract

As the two most principal active substances in the corn silk, polysaccharides and flavonoids, the mechanism of interaction between them has been a topic of intense research. This study provides an in-depth investigation of the interaction mechanism between corn silk glycans and luteoloside (LUT) and the synergistic role that result from this interaction. The interaction mechanism was evaluated by isothermal titration calorimetry (ITC) and circular dichroism (CD), and the synergistic role was evaluated by the expression of glucose transporters (GLUT-1), insulin secretion and surface plasmon resonance (SPR). CD and ITC results indicated that the interaction between CSGs and LUT mainly driven by the Cotton effects, enthalpy and entropy-driven. This interaction precipitated the formation of complexes (CSGs/LUT complexes) between corn silk glycans (CSGs) with four different molecular weights and luteoloside (LUT). Furthermore, the CSGs and LUT play a synergistic role in glucose regulation through GLUT-1 expression and insulin secretion experiments, compared to single luteoloside group.

Graphical Abstract

Abstract Image

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木犀草苷与玉米丝糖的相互作用机制及其在降血糖活性中的协同作用

多糖和黄酮类化合物是玉米丝中最主要的两种活性物质，它们之间的相互作用机理一直是研究的热点。本研究深入探讨了玉米丝聚糖与木犀草苷（LUT）之间的相互作用机制及其协同作用。通过等温滴定量热法（ITC）和圆二色法（CD）评估了相互作用机制，并通过葡萄糖转运体（GLUT-1）的表达、胰岛素分泌和表面等离子体共振（SPR）评估了协同作用。CD 和 ITC 结果表明，CSGs 和 LUT 之间的相互作用主要由 Cotton 效应、焓效应和熵效应驱动。这种相互作用使四种不同分子量的玉米丝聚糖（CSGs）与木犀草苷（LUT）之间形成了复合物（CSGs/LUT 复合物）。此外，通过 GLUT-1 表达和胰岛素分泌实验，CSGs 和 LUT 与单一的木犀草苷组相比，在葡萄糖调节中发挥了协同作用。

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来源期刊

Natural Products and Bioprospecting CHEMISTRY, MEDICINAL-

CiteScore

8.30

自引率

2.10%

发文量

审稿时长

13 weeks

期刊介绍： Natural Products and Bioprospecting serves as an international forum for essential research on natural products and focuses on, but is not limited to, the following aspects: Natural products: isolation and structure elucidation Natural products: synthesis Biological evaluation of biologically active natural products Bioorganic and medicinal chemistry Biosynthesis and microbiological transformation Fermentation and plant tissue cultures Bioprospecting of natural products from natural resources All research articles published in this journal have undergone rigorous peer review. In addition to original research articles, Natural Products and Bioprospecting publishes reviews and short communications, aiming to rapidly disseminate the research results of timely interest, and comprehensive reviews of emerging topics in all the areas of natural products. It is also an open access journal, which provides free access to its articles to anyone, anywhere.