{"title":"Taxonomy and nomenclature of Oophila amblystomatis (Chlorophyceae, Chlamydomonadales)","authors":"Cory D. Bishop, David J. Garbary","doi":"10.1111/jpy.13430","DOIUrl":null,"url":null,"abstract":"<p>The unicellular green alga <i>Oophila amblystomatis</i> was named by Lambert in 1905 based upon its association with egg masses of the spotted salamander <i>Ambystoma maculatum</i>. We collected algal cells from Lambert's original egg capsule preparations that were contributed to <i>Phycotheca Boreali-Americana</i> (PBA) in 1905 and subjected them to DNA extraction and PCR with <i>O. amblystomatis</i>-specific 18S rRNA gene primers. DNA amplified from these preparations was cloned and nine clones were sequenced. Along with representative sequences from the <i>Oophila</i> clade and Chlorophyceae, a phylogenetic tree was inferred. Seven sequences clustered within the <i>Oophila</i> clade and two clustered with <i>Chlamydomonas moewusii</i>, which is included in a sister clade to <i>Oophila</i>. By sequencing algal material from the egg capsules of representative type material we can unambiguously characterize <i>O. amblystomatis</i> and define a monophyletic clade centered on this type material. Accordingly, we reject a recent proposal that this species be transferred to <i>Chlorococcum</i>.</p>","PeriodicalId":16831,"journal":{"name":"Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Phycology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jpy.13430","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The unicellular green alga Oophila amblystomatis was named by Lambert in 1905 based upon its association with egg masses of the spotted salamander Ambystoma maculatum. We collected algal cells from Lambert's original egg capsule preparations that were contributed to Phycotheca Boreali-Americana (PBA) in 1905 and subjected them to DNA extraction and PCR with O. amblystomatis-specific 18S rRNA gene primers. DNA amplified from these preparations was cloned and nine clones were sequenced. Along with representative sequences from the Oophila clade and Chlorophyceae, a phylogenetic tree was inferred. Seven sequences clustered within the Oophila clade and two clustered with Chlamydomonas moewusii, which is included in a sister clade to Oophila. By sequencing algal material from the egg capsules of representative type material we can unambiguously characterize O. amblystomatis and define a monophyletic clade centered on this type material. Accordingly, we reject a recent proposal that this species be transferred to Chlorococcum.
期刊介绍：
The Journal of Phycology was founded in 1965 by the Phycological Society of America. All aspects of basic and applied research on algae are included to provide a common medium for the ecologist, physiologist, cell biologist, molecular biologist, morphologist, oceanographer, taxonomist, geneticist, and biochemist. The Journal also welcomes research that emphasizes algal interactions with other organisms and the roles of algae as components of natural ecosystems.
All aspects of basic and applied research on algae are included to provide a common medium for the ecologist, physiologist, cell biologist, molecular biologist, morphologist, oceanographer, acquaculturist, systematist, geneticist, and biochemist. The Journal also welcomes research that emphasizes algal interactions with other organisms and the roles of algae as components of natural ecosystems.
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