Liana L. Tevonyan, Artemy D. Beniaminov, Dmitry N. Kaluzhny
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引用次数: 0
Abstract
G-quadruplex (G4) structures formed by the guanine-rich DNA regions exhibit several distinctive optical properties, including UV absorption and circular dichroism spectra. Some G4 DNA possess intrinsic UV fluorescence whose origin is not completely clear to date. In this work, we study the effect of TMPyP4 and Methylene Blue on the intrinsic fluorescence of the dimeric G4 DNA structure formed by two d(G3T)4 sequences. We demonstrate that binding of the ligands results in quenching of the intrinsic fluorescence, although the conformation of the G4 DNA and its dimeric structure remain preserved. The binding sites of the ligands were suggested by the photoinduced oxidation of guanines and analysis of binding isoterms. We discuss how DNA-ligand complexes can affect the intrinsic fluorescence of G4 DNA.
摘要 由富含鸟嘌呤的 DNA 区域形成的 G-四重链（G4）结构具有多种独特的光学特性，包括紫外吸收和圆二色光谱。一些 G4 DNA 具有本征紫外荧光，但其来源至今尚未完全清楚。在这项工作中，我们研究了 TMPyP4 和亚甲蓝对由两个 d(G3T)4 序列形成的二聚 G4 DNA 结构本征荧光的影响。我们证明，虽然 G4 DNA 的构象及其二聚体结构保持不变，但配体的结合会导致本征荧光淬灭。配体的结合位点是通过鸟嘌呤的光诱导氧化和结合等值线分析得出的。我们讨论了 DNA 配体复合物如何影响 G4 DNA 的本征荧光。
期刊介绍：
The journal publishes papers in the field of biophysics, which is defined as the study of biological phenomena by using physical methods and concepts. Original papers, reviews and Biophysics letters are published. The primary goal of this journal is to advance the understanding of biological structure and function by application of the principles of physical science, and by presenting the work in a biophysical context.
Papers employing a distinctively biophysical approach at all levels of biological organisation will be considered, as will both experimental and theoretical studies. The criteria for acceptance are scientific content, originality and relevance to biological systems of current interest and importance.
Principal areas of interest include:
- Structure and dynamics of biological macromolecules
- Membrane biophysics and ion channels
- Cell biophysics and organisation
- Macromolecular assemblies
- Biophysical methods and instrumentation
- Advanced microscopics
- System dynamics.
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