The type VI secretion system of Stenotrophomonas rhizophila CFBP13503 limits the transmission of Xanthomonas campestris pv. campestris 8004 from radish seeds to seedlings

IF 4.8 1区农林科学 Q1 PLANT SCIENCES

Molecular plant pathology Pub Date : 2024-01-01 DOI:10.1111/mpp.13412

Tiffany Garin, Chrystelle Brin, Anne Préveaux, Agathe Brault, M. Briand, Marie Simonin, M. Barret, Laure Journet, A. Sarniguet

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引用次数: 0

Abstract

Abstract Stenotrophomonas rhizophila CFBP13503 is a seedborne commensal bacterial strain, which is efficiently transmitted to seedlings and can outcompete the phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc8004). The type VI secretion system (T6SS), an interference contact‐dependent mechanism, is a critical component of interbacterial competition. The involvement of the T6SS of S. rhizophila CFBP13503 in the inhibition of Xcc8004 growth and seed‐to‐seedling transmission was assessed. The T6SS cluster of S. rhizophila CFBP13503 and nine putative effectors were identified. Deletion of two T6SS structural genes, hcp and tssB, abolished the competitive advantage of S. rhizophila against Xcc8004 in vitro. The population sizes of these two bacterial species were monitored in seedlings after inoculation of radish seeds with mixtures of Xcc8004 and either S. rhizophila wild‐type (wt) strain or isogenic hcp mutant. A significant decrease in the population size of Xcc8004 was observed during confrontation with the S. rhizophila wt in comparison with T6SS‐deletion mutants in germinated seeds and seedlings. We found that the T6SS distribution among 835 genomes of the Stenotrophomonas genus is scarce. In contrast, in all available S. rhizophila genomes, T6SS clusters are widespread and mainly belong to the T6SS group i4. In conclusion, the T6SS of S. rhizophila CFBP13503 is involved in the antibiosis against Xcc8004 and reduces seedling transmission of Xcc8004 in radish. The distribution of this T6SS cluster in the S. rhizophila complex could make it possible to exploit these strains as biocontrol agents against X. campestris pv. campestris.

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根瘤黑僵菌 CFBP13503 的 VI 型分泌系统限制了野油菜黄单胞菌 8004 从萝卜种子向幼苗的传播

摘要根瘤黑僵菌（Stenotrophomonas rhizophila CFBP13503）是一种种子传播的共生细菌菌株，它能有效地传播给幼苗，并能与植物病原菌野油菜黄单胞菌（Xanthomonas campestris pv. campestris，Xcc8004）竞争。VI 型分泌系统（T6SS）是一种依赖于接触的干扰机制，是细菌间竞争的关键组成部分。本研究评估了根瘤菌 CFBP13503 的 T6SS 参与抑制 Xcc8004 的生长和种子到幼苗的传播。确定了根瘤蚜 CFBP13503 的 T6SS 簇和九个推定效应器。删除两个 T6SS 结构基因（hcp 和 tssB）后，根瘤菌在体外对 Xcc8004 的竞争优势消失。在用 Xcc8004 和根瘤菌野生型（wt）菌株或同源 hcp 突变体的混合物接种萝卜种子后，对幼苗中这两种细菌的种群数量进行了监测。与 T6SS 缺失突变体相比，在发芽种子和幼苗中，Xcc8004 与根瘤蚜野生型株对抗时，其种群数量明显减少。我们发现，T6SS 在臭单胞菌属 835 个基因组中的分布很少。相比之下，在所有可用的根瘤菌基因组中，T6SS 簇分布广泛，主要属于 T6SS i4 组。总之，根瘤嗜血杆菌 CFBP13503 的 T6SS 参与了对 Xcc8004 的抗生素作用，并减少了 Xcc8004 在萝卜中的幼苗传播。该 T6SS 簇在根瘤菌复合体中的分布使这些菌株有可能被用作抗野马毒素 X. campestris pv. campestris 的生物控制剂。

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来源期刊

Molecular plant pathology 生物-植物科学

CiteScore

9.40

自引率

4.10%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.

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