Effects of nano freezing-thawing on myofibrillar protein of Atlantic salmon fillets: Protein structure and label-free proteomics

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2024-01-14 DOI:10.1016/j.foodchem.2024.138369

Wenxuan Wang, Ying Bu, Wenzheng Li, Wenhui Zhu, Jianrong Li, Xuepeng Li

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Abstract

This study investigated the impact of magnetic nanoparticles (MNPs) -assisted cryogenic freezing integrated with MNPs combined microwave thawing (NNMT) on the structural integrity of myofibrillar proteins and alterations in protein profiles in salmon fillets. The NNMT showed the lowest myofibrillar fragmentation index (MFI) value (2.73 ± 0.31) among the four freezing-thawing groups. The myofibrillar structure exhibited the highest level of integrity, while the myofibrillar proteins demonstrated minimal aggregation and displayed the most stable secondary and tertiary structures in response to NNMT treatment. Compared with the other three treatments, NNMT exhibited a high abundance of ionic and hydrogen bonds, resulting in stronger interactions between the proteins and water molecules. The label-free proteomics analysis revealed that different freezing-thawing methods primarily affected the cytoskeletal proteins, with collagen and myosin being down-regulated due to degradation caused by cold stress and recrystallization. Additionally, NNMT demonstrated a superior capability in stabilizing salmon cytoskeletal proteins.

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纳米冷冻-解冻对大西洋鲑鱼片肌纤维蛋白的影响：蛋白质结构和无标记蛋白质组学

本研究调查了磁性纳米粒子（MNPs）辅助低温冷冻与磁性纳米粒子联合微波解冻（NNMT）对鲑鱼片中肌纤维蛋白结构完整性的影响以及蛋白质谱的改变。在四个冷冻-解冻组中，NNMT组的肌纤维碎裂指数（MFI）值最低（2.73 ± 0.31）。经 NNMT 处理后，肌原纤维结构的完整性水平最高，肌原纤维蛋白质的聚集程度最小，二级和三级结构最稳定。与其他三种处理相比，NNMT 表现出较高的离子键和氢键丰度，从而使蛋白质与水分子之间的相互作用更强。无标记蛋白质组学分析表明，不同的冻融方法主要影响细胞骨架蛋白，其中胶原蛋白和肌球蛋白因冷应力和重结晶引起的降解而下调。此外，NNMT 在稳定鲑鱼细胞骨架蛋白方面表现出了卓越的能力。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.