Phylogenetic Characterization of Resistant Salmonella Strains in Typhoid Fever Patients in Nigeria.

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS

Bioinformatics and Biology Insights Pub Date : 2024-01-09 eCollection Date: 2024-01-01 DOI:10.1177/11779322231220194

Olukayode Olugbenga Orole, Jebes Ngolo Lamini, Aleruchi Chuku

{"title":"Phylogenetic Characterization of Resistant Salmonella Strains in Typhoid Fever Patients in Nigeria.","authors":"Olukayode Olugbenga Orole, Jebes Ngolo Lamini, Aleruchi Chuku","doi":"10.1177/11779322231220194","DOIUrl":null,"url":null,"abstract":"Salmonella species are Enterobacteriaceae associated with typhoid fever. In this study, the distribution of broad-spectrum β-lactamase regulatory genes and genetic relatedness of isolates was determined. Stool samples (400) were collected from patients with fever in Dalhatu Araf Specialist Hospital (DASH), Lafia, Nigeria, between March 2020 and April 2021. Salmonella species were isolated and extended-spectrum β-lactamase distribution was determined among resistant isolates using polymerase chain reaction (PCR). Genetic relatedness of Salmonella species resistant to the 10 first-line antibiotics administered was determined among S typhi isolated. Of the 60 isolates that were confirmed to belong to the genus Salmonella, 12 (20.0%) isolates with bla SHV genes were the most prevalent, blaOXA-1 and blaCTX-M-9 were present in 5 isolates each, while blaCTX-M-4 and blaTEM genes with a prevalence of 1.7% each were the least obtained in the isolates. Two isolates had a multidrug-resistant index (MDRI) of 1, and 2 others were positive with the S typhi staG gene. Sequencing to determine their diversity showed that isolates ST36 and ST138, respectively, had MDRI = 1 and are clustered in a group with a similarity coefficient of 0.00634. The 2 isolates had the highest genetic similarity, which indicates that the genetic diversity between the isolates is low, while Salmonella strain ST313L2 had a high level of genetic distance from the other isolates. The most resistant isolates are closely related which calls for concern.","PeriodicalId":9065,"journal":{"name":"Bioinformatics and Biology Insights","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777790/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioinformatics and Biology Insights","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/11779322231220194","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

Salmonella species are Enterobacteriaceae associated with typhoid fever. In this study, the distribution of broad-spectrum β-lactamase regulatory genes and genetic relatedness of isolates was determined. Stool samples (400) were collected from patients with fever in Dalhatu Araf Specialist Hospital (DASH), Lafia, Nigeria, between March 2020 and April 2021. Salmonella species were isolated and extended-spectrum β-lactamase distribution was determined among resistant isolates using polymerase chain reaction (PCR). Genetic relatedness of Salmonella species resistant to the 10 first-line antibiotics administered was determined among S typhi isolated. Of the 60 isolates that were confirmed to belong to the genus Salmonella, 12 (20.0%) isolates with bla SHV genes were the most prevalent, blaOXA-1 and blaCTX-M-9 were present in 5 isolates each, while blaCTX-M-4 and blaTEM genes with a prevalence of 1.7% each were the least obtained in the isolates. Two isolates had a multidrug-resistant index (MDRI) of 1, and 2 others were positive with the S typhi staG gene. Sequencing to determine their diversity showed that isolates ST36 and ST138, respectively, had MDRI = 1 and are clustered in a group with a similarity coefficient of 0.00634. The 2 isolates had the highest genetic similarity, which indicates that the genetic diversity between the isolates is low, while Salmonella strain ST313L2 had a high level of genetic distance from the other isolates. The most resistant isolates are closely related which calls for concern.

查看原文本刊更多论文

尼日利亚伤寒患者耐药沙门氏菌菌株的系统发育特征。

沙门氏菌是与伤寒有关的肠杆菌科细菌。本研究确定了广谱β-内酰胺酶调控基因的分布以及分离菌株的遗传相关性。研究人员于 2020 年 3 月至 2021 年 4 月期间在尼日利亚拉菲亚的达尔哈图-阿拉夫专科医院（DASH）收集了发烧患者的粪便样本（400 份）。分离出沙门氏菌菌种，并利用聚合酶链式反应（PCR）确定耐药分离菌中扩展谱β-内酰胺酶的分布情况。在分离出的伤寒沙门氏菌中，确定了对 10 种一线抗生素耐药的沙门氏菌的基因亲缘关系。在确认属于沙门氏菌属的60个分离物中，12个（20.0%）分离物的 bla SHV 基因最为普遍，blaOXA-1 和 blaCTX-M-9 基因各出现在5个分离物中，而 blaCTX-M-4 和 blaTEM 基因在分离物中的出现率最低，各为1.7%。两个分离物的多重耐药指数（MDRI）为1，另外两个分离物的伤寒杆菌staG基因呈阳性。为确定其多样性而进行的测序显示，分离物 ST36 和 ST138 的 MDRI 分别为 1，并以 0.00634 的相似系数聚为一组。这两个分离株的遗传相似度最高，表明分离株之间的遗传多样性较低，而沙门氏菌株 ST313L2 与其他分离株的遗传距离较远。耐药性最强的分离物之间的亲缘关系密切，这一点值得关注。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bioinformatics and Biology Insights BIOCHEMICAL RESEARCH METHODS-

CiteScore

6.80

自引率

1.70%

发文量

审稿时长

8 weeks

期刊介绍： Bioinformatics and Biology Insights is an open access, peer-reviewed journal that considers articles on bioinformatics methods and their applications which must pertain to biological insights. All papers should be easily amenable to biologists and as such help bridge the gap between theories and applications.