Pathogenicity of Colletotrichum species isolated from rotten fruit and asymptomatic flowers of loquat in Nagasaki Prefecture, Japan and characterization of C. nagasakiense Takata & Kyoko Watan. sp. nov

IF 1 4区农林科学 Q3 PLANT SCIENCES

Journal of General Plant Pathology Pub Date : 2024-01-09 DOI:10.1007/s10327-023-01160-2

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引用次数: 0

Abstract

Species of Colletotrichum are common pathogens that cause loquat fruit rot in Nagasaki Prefecture, Japan. For clarifying the site of infection and the Colletotrichum species responsible for anthracnose in loquat trees, fungi were isolated from asymptomatic flowers and rotten fruits and identified to the species rank based on morphological and molecular phylogenetic analyses. Nineteen isolates from 148 rotten fruits were identified as C. fioriniae (12 isolates), C. nymphaeae (2 isolates), C. gloeosporioides s. s. (1 isolate), and four unidentified species of Colletotrichum. From 900 asymptomatic flowers, C. fioriniae (5 isolates), C. gloeosporioides s. s. (1 isolate), C. siamense (2 isolates), an unknown species (11 isolates) belonging to the C. acutatum species complex, and 2 were Colletotrichum spp. All isolates were pathogenic on wounded leaves and fruits. These results imply that C. fioriniae and C. gloeosporioides s. s. infect flowers and cause loquat fruit rot after maturation. However, the pathogenicity of the other species remains unclear as does the reason for the differences in the composition and proportions of fungal species in fruits and flowers. Eleven isolates obtained from asymptomatic flowers that also cause anthracnose disease in loquat were identified as a novel species of Colletotrichum, which was named C. nagasakiense sp. nov.

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从日本长崎县枇杷腐烂果实和无症状花朵中分离出的 Colletotrichum 菌种的致病性以及 C. nagasakiense Takata & Kyoko Watan.

摘要 Colletotrichum 是导致日本长崎县枇杷果实腐烂的常见病原体。为了明确枇杷树炭疽病的感染部位和病原菌种类，研究人员从无症状的花和腐烂的果实中分离出真菌，并根据形态学和分子系统学分析确定了真菌的种类。从 148 个腐烂的果实中分离出的 19 个分离物被鉴定为 C. fioriniae（12 个分离物）、C. nymphaeae（2 个分离物）、C. gloeosporioides s. s. （1 个分离物）和 4 个未确定种类的 Colletotrichum。从 900 朵无症状的花朵中，分离出了 C. fioriniae（5 株）、C. gloeosporioides s. s.（1 株）、C. siamense（2 株）、属于 C. acutatum 种类复合体的一个未知种类（11 株）和 2 株 Colletotrichum spp.，所有分离株都对受伤的叶片和果实具有致病性。这些结果表明，C. fioriniae 和 C. gloeosporioides s. s. 感染花朵并导致枇杷果实成熟后腐烂。然而，其他菌种的致病性以及果实和花朵中真菌种类的组成和比例差异的原因仍不清楚。从无症状的花朵中获得的 11 个分离物也会引起枇杷炭疽病，这些分离物被鉴定为 Colletotrichum 的一个新种，命名为 C. nagasakiense sp.

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来源期刊

Journal of General Plant Pathology PLANT SCIENCES-

CiteScore

2.40

自引率

8.30%

发文量

审稿时长

6 months

期刊介绍： The Journal of General Plant Pathology welcomes all manuscripts dealing with plant diseases or their control, including pathogen characterization, identification of pathogens, disease physiology and biochemistry, molecular biology, morphology and ultrastructure, genetics, disease transmission, ecology and epidemiology, chemical and biological control, disease assessment, and other topics relevant to plant pathological disorders.