WRR4B contributes to a broad-spectrum disease resistance against powdery mildew in Arabidopsis

IF 4.8 1区农林科学 Q1 PLANT SCIENCES

Molecular plant pathology Pub Date : 2024-01-08 DOI:10.1111/mpp.13415

Shuangshuang Mei, Yuxin Song, Zuer Zhang, Haitao Cui, Shuguo Hou, Weiguo Miao, Wei Rong

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Abstract

Oidium heveae HN1106, a powdery mildew (PM) that infects rubber trees, has been found to trigger disease resistance in Arabidopsis thaliana through ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)-, PHYTOALEXIN DEFICIENT 4 (PAD4)- and salicylic acid (SA)-mediated signalling pathways. In this study, a typical TOLL-INTERLEUKIN 1 RECEPTOR, NUCLEOTIDE-BINDING, LEUCINE-RICH REPEAT (TIR-NB-LRR)-encoding gene, WHITE RUST RESISTANCE 4 (WRR4B), was identified to be required for the resistance against O. heveae in Arabidopsis. The expression of WRR4B was upregulated by O. heveae inoculation, and WRR4B positively regulated the expression of genes involved in SA biosynthesis, such as EDS1, PAD4, ICS1 (ISOCHORISMATE SYNTHASE 1), SARD1 (SYSTEMIC-ACQUIRED RESISTANCE DEFICIENT 1) and CBP60g (CALMODULIN-BINDING PROTEIN 60 G). Furthermore, WRR4B triggered self-amplification, suggesting that WRR4B mediated plant resistance through taking part in the SA-based positive feedback loop. In addition, WRR4B induced an EDS1-dependent hypersensitive response in Nicotiana benthamiana and contributed to disease resistance against three other PM species: Podosphaera xanthii, Erysiphe quercicola and Erysiphe neolycopersici, indicating that WRR4B is a broad-spectrum disease resistance gene against PMs.

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WRR4B 促进拟南芥对白粉病的广谱抗病性

研究发现，感染橡胶树的白粉病（PM）Oidium heveae HN1106 可通过增强抗病性 1（EDS1）、植物抗毒素缺乏 4（PAD4）和水杨酸（SA）介导的信号通路触发拟南芥的抗病性。在这项研究中，一个典型的 TOLL-INTERLEUKIN 1 RECEPTOR、NUCLEOTIDE-BINDING、LEUCINE-RICH REPEAT（TIR-NB-LRR）编码基因--白锈抗性 4（WRR4B）被确定为拟南芥抗性 O. heveae 的必需基因。WRR4B的表达受O. heveae接种的上调，并且WRR4B正向调节参与SA生物合成的基因的表达，如EDS1、PAD4、ICS1（ISOCHORISMATE SYNTHASE 1）、SARD1（SYSTEMIC-ACCIRED RESISTANCE DEFICIENT 1）和CBP60g（CALMODULIN-BINDING PROTEIN 60 G）。此外，WRR4B 还引发了自我扩增，这表明 WRR4B 通过参与基于 SA 的正反馈环路来介导植物抗性。此外，WRR4B 还诱导了烟草中依赖 EDS1 的超敏反应，并促进了对其他三种 PM 的抗病性：Podosphaera xanthii、Erysiphe quercicola 和 Erysiphe neolycopersici。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular plant pathology 生物-植物科学

CiteScore

9.40

自引率

4.10%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.

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