Comparative Analysis of Green and Brown Morphotypes of Kappaphycus alvarezii Doty (Doty): Morphology, Total Phenol Content, Antioxidant Activity and Antimicrobial Activity

G.D.S.P. Rajapaksha, P. Gunathilake, B. Nirooparaj, J. K. Vidanarachchi, B. Jayawardana, M.D.S.T. De Croose, I. Wijesekara, P. C. G. Bandaranayake
{"title":"Comparative Analysis of Green and Brown Morphotypes of Kappaphycus alvarezii Doty (Doty): Morphology, Total Phenol Content, Antioxidant Activity and Antimicrobial Activity","authors":"G.D.S.P. Rajapaksha, P. Gunathilake, B. Nirooparaj, J. K. Vidanarachchi, B. Jayawardana, M.D.S.T. De Croose, I. Wijesekara, P. C. G. Bandaranayake","doi":"10.4038/tar.v35i1.8704","DOIUrl":null,"url":null,"abstract":"Kappaphycus alvarezii Doty (Doty), commonly known as elkhorn sea moss, is a popular seaweed cultivated globally. It is a well-known source of carrageenan, which is a thickening agent used in food and other industries. Even though different morphotypes of K. alvarezii are reported globally, it has been cultivated and marketed as a single genotype. However, such information is important, especially to produce high-value products such as nutraceuticals. Therefore, this study aimed to evaluate differences in microscopic structure, antimicrobial and antioxidant properties, and total phenolic contents of green and brown morphotypes of K. alvarezii cultivated in Jaffna, Sri Lanka. Except for the colour, there was no significant difference in macroscopic parameters. There was no significant difference observed in cross-sections under the light microscope. Antimicrobial properties of ethanol, methanol and water extracts were evaluated by disc diffusion assay against, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Antioxidant properties and total phenolic contents were determined using 1, 1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and Folin–Ciocalteau method, respectively. Interestingly, the antibacterial activity, antioxidant properties and total phenolic content of K. alvarezii depend on the morphotype. The green morphotype was rich in total phenolic compounds along with high antibacterial and antioxidant properties. Staphylococcus aureus was the most susceptible bacteria to antimicrobial agents of K. alvarezii and both ethanol and methanol extractions were efficient without significant differences between them in all the assays. The current data suggests better performance of the green morphotype of K. alvarezii as a natural source of antibacterial and antioxidant compounds.","PeriodicalId":23313,"journal":{"name":"Tropical agricultural research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical agricultural research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4038/tar.v35i1.8704","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Kappaphycus alvarezii Doty (Doty), commonly known as elkhorn sea moss, is a popular seaweed cultivated globally. It is a well-known source of carrageenan, which is a thickening agent used in food and other industries. Even though different morphotypes of K. alvarezii are reported globally, it has been cultivated and marketed as a single genotype. However, such information is important, especially to produce high-value products such as nutraceuticals. Therefore, this study aimed to evaluate differences in microscopic structure, antimicrobial and antioxidant properties, and total phenolic contents of green and brown morphotypes of K. alvarezii cultivated in Jaffna, Sri Lanka. Except for the colour, there was no significant difference in macroscopic parameters. There was no significant difference observed in cross-sections under the light microscope. Antimicrobial properties of ethanol, methanol and water extracts were evaluated by disc diffusion assay against, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Antioxidant properties and total phenolic contents were determined using 1, 1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and Folin–Ciocalteau method, respectively. Interestingly, the antibacterial activity, antioxidant properties and total phenolic content of K. alvarezii depend on the morphotype. The green morphotype was rich in total phenolic compounds along with high antibacterial and antioxidant properties. Staphylococcus aureus was the most susceptible bacteria to antimicrobial agents of K. alvarezii and both ethanol and methanol extractions were efficient without significant differences between them in all the assays. The current data suggests better performance of the green morphotype of K. alvarezii as a natural source of antibacterial and antioxidant compounds.
Kappaphycus alvarezii Doty(Doty)绿色和棕色形态的比较分析:形态、总酚含量、抗氧化活性和抗菌活性
Kappaphycus alvarezii Doty (Doty),俗称麋角海苔,是一种全球普遍种植的海藻。它是众所周知的卡拉胶来源,卡拉胶是一种用于食品和其他行业的增稠剂。尽管全球都有关于 K. alvarezii 不同形态型的报道,但它一直作为单一基因型进行栽培和销售。然而,这些信息非常重要,尤其是对生产营养保健品等高价值产品而言。因此,本研究旨在评估斯里兰卡贾夫纳栽培的 K. alvarezii 绿色和棕色形态的微观结构、抗菌和抗氧化特性以及总酚含量的差异。除颜色外,宏观参数无显著差异。在光学显微镜下观察横截面也无明显差异。乙醇、甲醇和水提取物的抗菌特性是通过盘扩散法对金黄色葡萄球菌、大肠杆菌和绿脓杆菌进行评估的。抗氧化性和总酚含量分别采用 1,1-二苯基-2-苦基肼(DPPH)自由基清除试验和 Folin-Ciocalteau 法进行测定。有趣的是,K. alvarezii 的抗菌活性、抗氧化性和总酚含量与形态有关。绿色形态富含总酚类化合物,具有较高的抗菌和抗氧化性。金黄色葡萄球菌是对 K. alvarezii 的抗菌剂最易感的细菌,乙醇和甲醇提取物在所有试验中都很有效,两者之间没有显著差异。目前的数据表明,作为抗菌和抗氧化化合物的天然来源,K. alvarezii 的绿色形态具有更好的性能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
审稿时长
16 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信