Morphological, Biochemical, and Molecular Diversity Assessment of Egyptian Bottle Gourd Cultivars

IF 1.4 4区生物学 Q4 GENETICS & HEREDITY

Genetics research Pub Date : 2024-01-03 DOI:10.1155/2024/4182158

Ehab A. Ibrahim, H. Alhaithloul, Sahar A. M. Shamseldin, S. B. Awaly, Abd El-Latif Hesham, Mohamed F. M. Abdelkader, M. M. Alqahtani, F. Alzuaibr, Abdulrahman Alasmari, Noha A. Sukar, Mohamed Z. Diyasty, M. A. Abdein

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Abstract

The genetic variability and relationships between ten bottle gourd cultivars were evaluated based on morphological, biochemical, and molecular parameters. The results displayed high variability among selected cultivars in terms of photosynthetic pigments, total free amino acids, total phenol content, isozymes pattern, and protein electrophoresis. Furthermore, differences in molecular markers were revealed by the SCoT technique. The peroxidase (POD) and polyphenyl oxidase (PPO) isozymes patterns did not detect significant differences in bands among cultivars. The protein patterns revealed seventeen bands ranging from 126 to 9 kDa and five polymorphic bands representing 29.41%. On the other hand, eight SCoT primers were used to evaluate the genetic variability and relationships between the ten Egyptian bottle gourd cultivars. The results of SCoT analysis detected 44 amplicons with 50% polymorphism. In addition, the results of the phylogenetic tree that is constructed based on the similarity coefficient revealed by SCoT analysis confirm the results of biochemical analysis indicating a genetic relationship between the most efficient bottle gourd cultivars (S1 and S2 cultivars). In addition, there is a genetic relationship among the less efficient bottle gourd cultivars (S4 and S5 cultivars). These results could be beneficial to distinguish among bottle gourd cultivars in the plant breeding programs.

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埃及瓶葫芦栽培品种的形态、生化和分子多样性评估

根据形态学、生物化学和分子参数评估了 10 个匏瓜栽培品种之间的遗传变异性和关系。结果表明，在光合色素、总游离氨基酸、总酚含量、同工酶模式和蛋白质电泳方面，所选栽培品种之间存在很大差异。此外，SCoT 技术还显示了分子标记的差异。过氧化物酶（POD）和多苯基氧化酶（PPO）同工酶模式在不同栽培品种之间没有发现明显的条带差异。蛋白质模式显示出 17 条从 126 到 9 kDa 的条带和 5 条多态条带，占 29.41%。另一方面，使用八种 SCoT 引物评估了十个埃及瓶瓠栽培品种之间的遗传变异性和遗传关系。SCoT 分析结果检测到 44 个扩增子，多态性为 50%。此外，根据 SCoT 分析揭示的相似系数构建的系统发生树的结果证实了生化分析的结果，表明最高效的瓶瓠栽培品种（S1 和 S2 栽培品种）之间存在遗传关系。此外，效率较低的瓶瓠栽培品种（S4 和 S5 栽培品种）之间也存在遗传关系。这些结果有助于在植物育种计划中区分瓶瓠栽培品种。

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来源期刊

Genetics research 生物-遗传学

自引率

6.70%

发文量

审稿时长

>12 weeks

期刊介绍： Genetics Research is a key forum for original research on all aspects of human and animal genetics, reporting key findings on genomes, genes, mutations and molecular interactions, extending out to developmental, evolutionary, and population genetics as well as ethical, legal and social aspects. Our aim is to lead to a better understanding of genetic processes in health and disease. The journal focuses on the use of new technologies, such as next generation sequencing together with bioinformatics analysis, to produce increasingly detailed views of how genes function in tissues and how these genes perform, individually or collectively, in normal development and disease aetiology. The journal publishes original work, review articles, short papers, computational studies, and novel methods and techniques in research covering humans and well-established genetic organisms. Key subject areas include medical genetics, genomics, human evolutionary and population genetics, bioinformatics, genetics of complex traits, molecular and developmental genetics, Evo-Devo, quantitative and statistical genetics, behavioural genetics and environmental genetics. The breadth and quality of research make the journal an invaluable resource for medical geneticists, molecular biologists, bioinformaticians and researchers involved in genetic basis of diseases, evolutionary and developmental studies.